POMT2 Antikörper

Produktnummer ABIN6719505

Dieses Anti-POMT2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von POMT2 in WB, IHC, FACS, ICC und IF. Geeignet für Human, Maus und Ratte.

Kurzübersicht für POMT2 Antikörper (ABIN6719505)

Target: POMT2 (Protein-O-Mannosyltransferase 2 (POMT2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POMT2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-POMT2 Antikörper

Verwendungszweck: Anti-POMT2 Antibody Picoband®

Sequenz: QAARAAGRDV AAEAVARSPK R

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-POMT2 Antibody Picoband® (ABIN6719505). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human POMT2, which shares 61.9% amino acid (aa) sequence identity with both mouse and rat POMT2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1. Biancheri, R., Falace, A., Tessa, A., Pedemonte, M., Scapolan, S., Cassandrini, D., Aiello, C., Rossi, A., Broda, P., Zara, F., Santorelli, F. M., Minetti, C., Bruno, C. POMT2 gene mutation in limb-girdle muscular dystrophy with inflammatory changes. Biochem. Biophys. Res. Commun. 363: 1033-1037, 2007. 2. Mercuri, E., D'Amico, A., Tessa, A., Berardinelli, A., Pane, M., Messina, S., van Reeuwijk, J., Bertini, E., Muntoni, F., Santorelli, F. M. POMT2 mutation in a patient with 'MEB-like' phenotype. Neuromusc. Disord. 16: 446-448, 2006.

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu POMT2

Target: POMT2 (Protein-O-Mannosyltransferase 2 (POMT2))

Andere Bezeichnung: POMT2

Hintergrund:

Synonyms: Protein O-mannosyl-transferase 2, Dolichyl-phosphate-mannose--protein mannosyltransferase 2, POMT2

Tissue Specificity: Highly expressed in testis, detected at low levels in most tissues.

Background: Protein O-mannosyl-transferase 2 is an enzyme that in humans is encoded by the POMT2 gene. This gene is mapped to chromosome 14q24.3. The protein encoded by this gene is an O-mannosyltransferase that requires interaction with the product of the POMT1 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of Walker-Warburg syndrome (WWS).

Molekulargewicht: 84 kDa

Gen-ID: 29954