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SNAP25 Antikörper (AA 1-203)

Der Kaninchen Polyklonal Anti-SNAP25-Antikörper wurde für WB, ELISA, IHC, IF, ICC und FACS validiert. Er ist geeignet, SNAP25 in Proben von Human, Ratte und Maus zu detektieren.
Produktnummer ABIN6719447

Kurzübersicht für SNAP25 Antikörper (AA 1-203) (ABIN6719447)

Target

Alle SNAP25 Antikörper anzeigen
SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reaktivität

  • 103
  • 76
  • 70
  • 10
  • 10
  • 9
  • 8
  • 8
  • 7
  • 6
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Ratte, Maus

Wirt

  • 104
  • 24
  • 4
  • 3
  • 1
Kaninchen

Klonalität

  • 100
  • 33
  • 1
Polyklonal

Konjugat

  • 80
  • 8
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser SNAP25 Antikörper ist unkonjugiert

Applikation

  • 97
  • 47
  • 34
  • 22
  • 19
  • 18
  • 13
  • 13
  • 13
  • 12
  • 8
  • 5
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
  • Bindungsspezifität

    • 18
    • 15
    • 12
    • 8
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-203

    Verwendungszweck

    Anti-SNAP25 Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-SNAP25 Antibody Picoband® (ABIN6719447). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E. coli-derived human SNAP25 recombinant protein (Position:M1-L203).

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    Immunocytochemistry/Immunofluorescence, 5 μg/mL
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells
    ELISA, 0.1-0.5 μg/mL
    1. Gonelle-Gispert, C., Halban, P. A., Niemann, H., Palmer, M., Catsicas, S., Sadoul, K. : SNAP-25a and -25b isoforms are both expressed in insulin-secreting cells and can function in insulin secretion. Biochem. J. 339: 159-165, 1999. 2. Nagy, G., Reim, K., Matti, U., Brose, N., Binz, T., Rettig, J., Neher, E., Sorensen, J. B. : Regulation of releasable vesicle pool sizes by protein kinase A-dependent phosphorylation of SNAP-25. Neuron 41: 417-429, 2004. 3. Hodel A (1998). "SNAP-25". The International Journal of Biochemistry & Cell Biology 30 (10): 1069-1073. 4. Chapman ER (2002). "Synaptotagmin: A Ca2+ sensor that triggers exocytosis?". Nature Reviews Molecular Cell Biology 3: 498-508. 5. Pozzi D, Verderio C, Patti L, Grumelli C, Inverardi F, Frassoni C, Bonanno G, Matteoli M (2004). "SNAP-25 modulation of calcium dynamics underlies differences in GABAergic and glutamatergic responsiveness to depolarization". Neuron 41 (4): 599-610.

    Kommentare

    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

    Andere Bezeichnung

    SNAP25

    Hintergrund

    Synonyms: Synaptosomal-associated protein 25, SNAP-25, Super protein, SUP, Synaptosomal-associated 25 kDa protein, SNAP25, SNAP

    Tissue Specificity: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.

    Background: Synaptosome-associated protein of 25,000 daltons, also known as SNAP-25, is a protein which in humans encodes a 25-kD protein of 206 amino acids. It was first investigated as a neuron-specific gene preferentially expressed in mouse hippocampus. The tSNARE (the target-membrane soluble NSF-attachment protein receptor, where NSF is N-ethylmaleimide-sensitive fusion protein) synaptosomal-associated protein of 25 kDa (SNAP-25) is expressed in pancreatic B-cells and its cleavage by botulinum neurotoxin E (BoNT/E) abolishes stimulated secretion of insulin. In the nervous system, two SNAP-25 isoforms (a and b) have been described, which are produced by alternative splicing. It is identified mammalian Snap25a and Snap25b as targets of protein kinase A, a key regulator of neurosecretion that primes slowly releasable pools and readily releasable pools of secretory vesicles. SNAP-25 inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP-25 decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.

    Molekulargewicht

    25 kDa

    UniProt

    P60880

    Pathways

    Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
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