Golgin A2 (GOLGA2) (AA 796-913) Antikörper

Produktnummer ABIN6719432

Der Maus Monoklonal Anti--Antikörper wurde für WB, IHC, IF, ICC und FACS validiert. Er ist geeignet, in Proben von Human zu detektieren.

Kurzübersicht für Golgin A2 (GOLGA2) (AA 796-913) Antikörper (ABIN6719432)

Target: Golgin A2 (GOLGA2)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: 4G3

Produktdetails

Bindungsspezifität: AA 796-913

Verwendungszweck: Anti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 4G3)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti- GOLGA2 Antibody Picoband® (monoclonal, 4G3) (ABIN6719432). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human GM130 recombinant protein (Position: E796-E913).

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1.Matsuki, T., Matthews, R. T., Cooper, J. A., van der Brug, M. P., Cookson, M. R., Hardy, J. A., Olson, E. C., Howell, B. W. Reelin and Stk25 have opposing roles in neuronal polarization and dendritic Golgi deployment. Cell 143: 826-836, 2010.  Wong, M., Munro, S. The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins. Science 346: 601 only, 2014.

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Antigendetails

Target: Golgin A2 (GOLGA2)

Andere Bezeichnung: GOLGA2

Hintergrund:

Synonyms: Golgin subfamily A member 2, 130 kDa cis-Golgi matrix protein, GM130, GM130 autoantigen, Golgin-95, GOLGA2

Tissue Specificity: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Background: Golgin subfamily A member 2 is a protein that in humans is encoded by the GOLGA2 gene. The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.

Molekulargewicht: 140 kDa

Gen-ID: 2801

UniProt: Q08379

Pathways: SARS-CoV-2 Protein Interaktom