DOK7 Antikörper

Produktnummer ABIN6719428

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch DOK7 in WB, IHC und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für DOK7 Antikörper (ABIN6719428)

Target: DOK7 (Docking Protein 7 (DOK7))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DOK7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-DOK7 Antikörper

Verwendungszweck: Anti-DOK7 Antibody Picoband®

Sequenz: STVEERVAQE ALETLQLEKR LSLLSHAGR

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-DOK7 Antibody Picoband® (ABIN6719428). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human DOK7, which shares 86.2% amino acid (aa) sequence identity with mouse DOK7.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells
1. Arimura, S., Okada, T., Tezuka, T., Chiyo, T., Kasahara, Y., Yoshimura, T., Motomura, M., Yoshida, N., Beeson, D., Takeda, S., Yamanashi, Y. DOK7 gene therapy benefits mouse models of diseases characterized by defects in the neuromuscular junction. Science 345: 1505-1508, 2014. 2. Beeson, D., Higuchi, O., Palace, J., Cossins, J., Spearman, H., Maxwell, S., Newsom-Davis, J., Burke, G., Fawcett, P., Motomura, M., Muller, J. S., Lochmuller, H., Slater, C., Vincent, A., Yamanashi, Y. Dok-7 mutations underlie a neuromuscular junction synaptopathy. Science 313: 1975-1978, 2006. 3. Hallock, P. T., Xu, C.-F., Park, T.-J., Neubert, T. A., Curran, T., Burden, S. J. Dok-7 regulates neuromuscular synapse formation by recruiting Crk and Crk-L. Genes Dev. 24: 2451-2461, 2010.

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu DOK7

Target: DOK7 (Docking Protein 7 (DOK7))

Andere Bezeichnung: DOK7

Hintergrund:

Synonyms: Protein Dok-7, Downstream of tyrosine kinase 7, DOK7, C4orf25,

Tissue Specificity: Preferentially expressed in skeletal muscle and heart. Present in thigh muscle, diaphragm and heart but not in the liver or spleen .

Background: Dok-7 is a non-catalytic cytoplasmic adaptor protein that is expressed specifically in muscle and is essential for the formation of neuromuscular synapses. Further, Dok-7 contains pleckstrin homology (PH) and phosphotyrosine-binding (PTB) domains that are critical for Dok-7 function. It is mapped to 4p16.3. The protein encoded by this gene is essential for neuromuscular synaptogenesis. The protein functions in aneural activation of muscle-specific receptor kinase, which is required for postsynaptic differentiation, and in the subsequent clustering of the acetylcholine receptor in myotubes. This protein can also induce autophosphorylation of muscle-specific receptor kinase. Mutations in this gene are a cause of familial limb-girdle myasthenia autosomal recessive, which is also known as congenital myasthenic syndrome type 1B. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 65 kDa

Gen-ID: 285489

Pathways: Skeletal Muscle Fiber Development