AIP Antikörper (AA 91-330)

Produktnummer ABIN6719423

Der Maus Monoklonal Anti-AIP-Antikörper wurde für WB, IHC, ICC, IF und FACS validiert. Er ist geeignet, AIP in Proben von Human, Maus, Ratte und Affe zu detektieren.

Kurzübersicht für AIP Antikörper (AA 91-330) (ABIN6719423)

Target: AIP (Aryl Hydrocarbon Receptor Interacting Protein (AIP))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser AIP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 10G8

Produktdetails anti-AIP Antikörper

Bindungsspezifität: AA 91-330

Verwendungszweck: Anti-ARA9/AIP Antibody Picoband® (monoclonal, 10G8)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-ARA9/AIP Antibody Picoband® (monoclonal, 10G8) (ABIN6719423). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human ARA9 recombinant protein (Position: D91-H330). Human ARA9 shares 95% amino acid (aa) sequence identity with both mouse and rat ARA9.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1. Carver LA, Bradfield CA (April 1997). "Ligand-dependent interaction of the aryl hydrocarbon receptor with a novel immunophilin homolog in vivo". J. Biol. Chem. 272(17): 11452-6. 2. Kuzhandaivelu, N., Cong, Y.-S., Inouye, C., Yang, W.-M., Seto, E. XAP2, a novel hepatitis B virus X-associated protein that inhibits X transactivation. Nucleic Acids Res. 24: 4741-4750, 1996. 3. Occhi G, Trivellin G, Ceccato F, et al. (2010). "Prevalence of AIP mutations in a large series of sporadic Italian acromegalic patients and evaluation of CDKN1B status in acromegalic patients with multiple endocrine neoplasia.". Eur. J. Endocrinol. 163 (3): 369-376.

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu AIP

Target: AIP (Aryl Hydrocarbon Receptor Interacting Protein (AIP))

Andere Bezeichnung: AIP

Hintergrund:

Synonyms: H receptor-interacting protein, AIP, Aryl-hydrocarbon receptor-interacting protein, HBV X-associated protein 2, XAP-2, Immunophilin homolog ARA9, AIP, XAP2

Tissue Specificity: Widely expressed. Higher levels seen in the heart, placenta and skeletal muscle. Not expressed in the liver.

Background: AIP, also known as, ARA9 or XAP-2, is a protein that in humans is encoded by the AIP gene. This gene is mapped to 11q13.2. The encoded protein is found in the cytoplasm as part of a multiprotein complex, but upon binding of ligand is transported to the nucleus. AIP may play a positive role in aryl hydrocarbon receptor-mediated signalling possibly by influencing its receptivity for ligand and/or its nuclear targeting. It has been shown that AIP is the cellular negative regulator of the hepatitis B virus (HBV) X protein. AIP mutations may be the cause of a familial form of acromegaly, familial isolated pituitary adenoma (FIPA).

Molekulargewicht: 38 kDa

Gen-ID: 9049

UniProt: O00170