ITGA3 Antikörper (N-Term)

Produktnummer ABIN6719410

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch ITGA3 in WB, IHC, FACS, ICC, IF und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für ITGA3 Antikörper (N-Term) (ABIN6719410)

Target: ITGA3 (Integrin, alpha 3 (ITGA3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ITGA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-ITGA3 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Anti-Integrin alpha 3/ITGA3 Antibody

Sequenz: YSVALHRQTE RQQRYLLLAG APRELAVPDG YTNR

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Integrin alpha 3/ITGA3 Antibody. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Integrin alpha 3, which shares 91.2% amino acid (aa) sequence identity with both mouse and rat Integrin alpha 3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL Immunohistochemistry (Frozen Section), 0.5-1 μg/mL Immunocytochemistry/Immunofluorescence, 2 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu ITGA3

Target: ITGA3 (Integrin, alpha 3 (ITGA3))

Andere Bezeichnung: ITGA3

Hintergrund:

Background: ITGA3 (INTEGRIN, ALPHA-3), also called CD49C, VLA3 or GAPB3, is a protein that in humans is encoded by the ITGA3 gene. It is an integrin alpha subunit which is also a member of the family of cell surface adhesion molecules. This gene is mapped to chromosome 17 and its exact cytogenetic location is 17q21.33. ITGA3 makes up half of the α3β1 integrin duplex that plays a role in neural migration and corticogenesis together with beta-1 subunit.A functional link between DAB1 phosphorylation and ITGA3 signaling drives the timely detachment of migrating neurons from radial glial fibers. Expression of human ITGA3 increased the infectivity of virus for Chinese hamster ovary cells. ITGA3 also contains 13 potential N-glycosylation sites, 2 potential cleavage sites, and the 7 N-terminal repeating units characteristic of ITGAs. Recombinant ITGA3 is expressed as a 150-kD protein as the same size as the native protein by the western blot analysis.

Gene Full Name: integrin subunit alpha 3

Molekulargewicht: 130 kDa

Gen-ID: 3675

UniProt: P26006

Pathways: CXCR4-mediated Signaling Events, Integrin Complex