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HERC5 Antikörper (AA 793-1024)

Der Kaninchen Polyklonal Anti-HERC5-Antikörper wurde für WB, ELISA, IF und ICC validiert. Er ist geeignet, HERC5 in Proben von Human zu detektieren.
Produktnummer ABIN6719405

Kurzübersicht für HERC5 Antikörper (AA 793-1024) (ABIN6719405)

Target

Alle HERC5 Antikörper anzeigen
HERC5 (Hect Domain and RLD 5 (HERC5))

Reaktivität

  • 14
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 13
  • 1
Kaninchen

Klonalität

  • 14
Polyklonal

Konjugat

  • 11
  • 1
  • 1
  • 1
Dieser HERC5 Antikörper ist unkonjugiert

Applikation

  • 9
  • 5
  • 5
  • 2
  • 2
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 793-1024

    Verwendungszweck

    Anti-HERC5 Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-HERC5 Antibody Picoband® (ABIN6719405). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human HERC5 recombinant protein (Position: F793-G1024).

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL
    Immunocytochemistry/Immunofluorescence, 5 μg/mL
    ELISA, 0.1-0.5 μg/mL
    1. Hochrainer, K., Mayer, H., Baranyi, U., Binder, B. R., Lipp, J., Kroismayr, R. The human HERC family of ubiquitin ligases: novel members, genomic organization, expression profiling, and evolutionary aspects. Genomics 85: 153-164, 2005. 2. Mitsui, K., Nakanishi, M., Ohtsuka, S., Norwood, T. H., Okabayashi, K., Miyamoto, C., Tanaka, K., Yoshimura, A., Ohtsubo, M. A novel human gene encoding HECT domain and RCC1-like repeats interacts with cyclins and is potentially regulated by the tumor suppressor proteins. Biochem. Biophys. Res. Commun. 266: 115-122, 1999. 3. Wong, J. J. Y., Pung, Y. F., Sze, N. S.-K., Chin, K.-C. HERC5 is an IFN-induced HECT-type E3 protein ligase that mediates type I IFN-induced ISGylation of protein targets. Proc. Nat. Acad. Sci. 103: 10735-10740, 2006.

    Kommentare

    Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    HERC5 (Hect Domain and RLD 5 (HERC5))

    Andere Bezeichnung

    HERC5

    Hintergrund

    Synonyms: E3 ISG15--protein ligase HERC5, Cyclin-E-binding protein 1, HECT domain and RCC1-like domain-containing protein 5, HERC5, CEB1, CEBP1

    Tissue Specificity: Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.

    Background: Probable E3 ubiquitin-protein ligase HERC5 is an enzyme that in humans is encoded by the HERC5 gene. It is mapped to 4q22.1. This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets.

    Molekulargewicht

    117 kDa

    Gen-ID

    51191
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