MID1 Antikörper (AA 226-278)

Produktnummer ABIN6719400

Produktdetails anti-MID1 Antikörper

Target: MID1 (Midline 1 (MID1))

Bindungsspezifität: AA 226-278

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MID1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for MID1 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for MID1 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human MID1 recombinant protein (Position: N226-Q278).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Direct ELISA|0.1-0.5 μg/mL

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu MID1

Target: MID1 (Midline 1 (MID1))

Andere Bezeichnung: MID1 (MID1 Produkte)

Synonyme: BBBG1 antikoerper, FXY antikoerper, GBBB1 antikoerper, MIDIN antikoerper, OGS1 antikoerper, OS antikoerper, OSX antikoerper, RNF59 antikoerper, TRIM18 antikoerper, XPRF antikoerper, ZNFXY antikoerper, 61B3-R antikoerper, DXHXS1141 antikoerper, Fxy antikoerper, Trim18 antikoerper, Midline1 antikoerper, bbbg1 antikoerper, fxy antikoerper, gbbb1 antikoerper, midin antikoerper, ogs1 antikoerper, osx antikoerper, rnf59 antikoerper, trim18 antikoerper, xprf antikoerper, znfxy antikoerper, midline 1 antikoerper, midline 1 L homeolog antikoerper, MID1 antikoerper, Mid1 antikoerper, mid1 antikoerper, mid1.L antikoerper

Hintergrund:

Synonyms: E3 ubiquitin-protein ligase Midline-1, Midin, Putative transcription factor XPRF, RING finger protein 59, RING finger protein Midline-1, RING-type E3 ubiquitin transferase Midline-1, Tripartite motif-containing protein 18, MID1, FXY, RNF59, TRIM18, XPRF

Background: Midline-1 is a protein found in humans that is encoded by the MID1 gene. The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities.

Gen-ID: 4281

UniProt: O15344