RNF123 Antikörper (AA 12-188)

Produktnummer ABIN6719387

Der Kaninchen Polyklonal Anti-RNF123-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, RNF123 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für RNF123 Antikörper (AA 12-188) (ABIN6719387)

Target: RNF123 (Ring Finger Protein 123 (RNF123))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RNF123 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-RNF123 Antikörper

Bindungsspezifität: AA 12-188

Verwendungszweck: Anti-RNF123 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-R Antibody Picoband® (ABIN6719387). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human RNF123 recombinant protein (Position: R12-Y188).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Kamura, T., Hara, T., Matsumoto, M., Ishida, N., Okumura, F., Hatakeyama, S., Yoshida, M., Nakayama, K., Nakayama, K. I. Cytoplasmic ubiquitin ligase KPC regulates proteolysis of p27(Kip1) at G1 phase. Nature Cell Biol. 6: 1229-1235, 2004. 2. Lu, Y., Adegoke, O. A. J., Nepveu, A., Nakayama, K. I., Bedard, N., Cheng, D., Peng, J., Wing, S. S. USP19 deubiquitinating enzyme supports cell proliferation by stabilizing KPC1, a ubiquitin ligase for p27(Kip1). Molec. Cell. Biol. 29: 547-558, 2009. Note: Erratum: Molec. Cell. Biol. 29: 3241 only, 2009.

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu RNF123

Target: RNF123 (Ring Finger Protein 123 (RNF123))

Andere Bezeichnung: RNF123

Hintergrund:

Synonyms: E3 ubiquitin-protein ligase RNF123, Kip1 ubiquitination-promoting complex protein 1, RING finger protein 123, RING-type E3 ubiquitin transferase RNF123, RNF123, KPC1, FP1477

Tissue Specificity: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.

Background: E3 ubiquitin-protein ligase RNF123 is an enzyme that in humans is encoded by the RNF123 gene. It is mapped to 3p21.31. The protein encoded by this gene contains a C-terminal RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions, and an N-terminal SPRY domain. This protein displays E3 ubiquitin ligase activity toward the cyclin-dependent kinase inhibitor 1B which is also known as p27 or KIP1. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 149 kDa

Gen-ID: 63891