NFIA Antikörper (Middle Region)

Produktnummer ABIN6719349

Der Maus Monoklonal anti-NFIA Antikörper (Klon 16H11) (ABIN6719349) detektiert spezifisch NFIA in WB, IHC, IF, ICC und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für NFIA Antikörper (Middle Region) (ABIN6719349)

Target: NFIA (Nuclear Factor I/A (NFIA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NFIA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: 16H11

Produktdetails anti-NFIA Antikörper

Bindungsspezifität: AA 180-224, Middle Region

Verwendungszweck: Anti-NFIA Antibody (monoclonal, 16H11)

Sequenz: AYFVHAADSS QSESPSQPSD ADIKDQPENG HLGFQDSFVT SGVFS

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NFIA Antibody (monoclonal, 16H11) (ABIN6719349). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human NFIA, different from the related mouse sequence by one amino acid, and identical to the related rat sequence.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu NFIA

Target: NFIA (Nuclear Factor I/A (NFIA))

Andere Bezeichnung: NFIA

Hintergrund:

Synonyms: Nuclear factor 1 A-type, NF1-A, Nuclear factor 1/A, CCAAT-box-binding transcription factor, CTF, Nuclear factor I/A, NF-I/A, NFI-A, TGGCA-binding protein, NFIA, KIAA1439

Tissue Specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.

Background: Nuclear factor 1 A-type?is a?protein?that in?humans?is encoded?by the?NFIA?gene. Nuclear factor I (NFI) proteins constitute a family of dimericDNA-binding proteins?with similar, and possibly identical, DNA-binding specificity. They function as cellular?transcription factors?and as replication factors for?adenovirus?DNA replication. Diversity in this protein family is generated by multiple?genes, differential?splicing, and heterodimerization.

Molekulargewicht: 62 kDa

Gen-ID: 4774

UniProt: Q12857