CST3 Antikörper (AA 31-146)

Produktnummer ABIN6719299

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CST3 in WB, ELISA, IHC, FACS, IF und ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CST3 Antikörper (AA 31-146) (ABIN6719299)

Target: CST3 (Cystatin C (CST3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CST3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 4H8

Produktdetails anti-CST3 Antikörper

Bindungsspezifität: AA 31-146

Verwendungszweck: Anti-Cystatin C/CST3 Antibody Picoband® (monoclonal, 4H8)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Cystatin C/CST3 Antibody Picoband® (monoclonal, 4H8) (ABIN6719299). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human Cystatin C recombinant protein (Position: K31-A146).

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA (Cap), 1-5 μg/mL
1. Abrahamson, M., Grubb, A. Increased body temperature accelerates aggregation of the leu68-to-gln mutant cystatin C, the amyloid-forming protein in hereditary cystatin C amyloid angiopathy. Proc. Nat. Acad. Sci. 91: 1416-1420, 1994. 2. Crawford, F. C., Freeman, M. J., Schinka, J. A., Abdullah, L. I., Gold, M., Hartman, R., Krivian, K., Morris, M. D., Richards, D., Duara, R., Anand, R., Mullan, M. J. A polymorphism in the cystatin C gene is a novel risk factor for late-onset Alzheimer's disease. Neurology 55: 763-768, 2000.

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu CST3

Target: CST3 (Cystatin C (CST3))

Andere Bezeichnung: CST3

Hintergrund:

Synonyms: Nuclear factor 1 A-type, NF1-A, Nuclear factor 1/A, CCAAT-box-binding transcription factor, CTF, Nuclear factor I/A, NF-I/A, NFI-A, TGGCA-binding protein, NFIA, KIAA1439

Tissue Specificity: Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.

Background: Cystatin C or cystatin 3, a protein encoded by the CST3 gene, is mainly used as a biomarker of kidney function. Recently, it has been studied for its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer's disease. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and body fluids. It is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Cystatin C belongs to the type 2 cystatin gene family.

Molekulargewicht: 15 kDa

Gen-ID: 1471

UniProt: P01034