DARS2 Antikörper (AA 334-448)

Produktnummer ABIN6719279

Der Kaninchen Polyklonal anti-DARS2 Antikörper wird verwendet zum Nachweis von DARS2 in Proben von Human. Er wurde validiert für WB, IHC, ELISA, ICC, FACS und IF.

Kurzübersicht für DARS2 Antikörper (AA 334-448) (ABIN6719279)

Target: DARS2 (Aspartyl-tRNA Synthetase 2, Mitochondrial (DARS2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DARS2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails anti-DARS2 Antikörper

Bindungsspezifität: AA 334-448

Verwendungszweck: Anti-DARS2 Antibody

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-DARS2 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human DARS2 recombinant protein (Position: D334-A448).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL Immunocytochemistry/Immunofluorescence, 2 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells ELISA, 0.1-0.5 μg/mL

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu DARS2

Target: DARS2 (Aspartyl-tRNA Synthetase 2, Mitochondrial (DARS2))

Andere Bezeichnung: DARS2

Hintergrund:

Background: DARS2 contains conserved residues involved in ATP binding, tRNA binding, and aspartic acid recognition, as well as catalytic site motifs characteristic of amino acid tRNA synthetases. The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). The International Radiation Hybrid Mapping Consortium mapped the DARS2 gene to chromosome 1.

Gene Full Name: aspartyl-tRNA synthetase 2, mitochondrial

Molekulargewicht: 74 kDa

Gen-ID: 55157

UniProt: Q6PI48