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ADA Antikörper (AA 135-363)

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ADA in WB, IHC und FACS. Er zeigt eine Reaktivität gegenüber Human.
Produktnummer ABIN6719272

Kurzübersicht für ADA Antikörper (AA 135-363) (ABIN6719272)

Target

Alle ADA Antikörper anzeigen
ADA (Adenosine Deaminase (ADA))

Reaktivität

  • 65
  • 35
  • 19
  • 14
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Human

Wirt

  • 76
  • 19
  • 1
Maus

Klonalität

  • 78
  • 18
Monoklonal

Konjugat

  • 52
  • 8
  • 6
  • 6
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser ADA Antikörper ist unkonjugiert

Applikation

  • 85
  • 42
  • 15
  • 14
  • 13
  • 13
  • 13
  • 11
  • 10
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Klon

6D4
  • Bindungsspezifität

    • 15
    • 9
    • 8
    • 8
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 135-363

    Verwendungszweck

    Anti-ADA Antibody Picoband® (monoclonal, 6D4)

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-ADA Antibody Picoband® (monoclonal, 6D4) (ABIN6719272). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E. coli-derived human ADA recombinant protein (Position: Q135-L363). Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively.

    Isotyp

    IgG2b
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells
    1. Blackburn MR (2003). "Too much of a good thing: adenosine overload in adenosine-deaminase-deficient mice". Trends in Pharmacological Sciences 24 (2): 66-70. 2. Cristalli G, Costanzi S, Lambertucci C, Lupidi G, Vittori S, Volpini R, Camaioni E (Mar 2001). "Adenosine deaminase: functional implications and different classes of inhibitors". Medicinal Research Reviews 21 (2): 105-128. 3. Wilson DK, Rudolph FB, Quiocho FA (May 1991). "Atomic structure of adenosine deaminase complexed with a transition-state analog: understanding catalysis and immunodeficiency mutations". Science 252(5010): 1278-1284.

    Kommentare

    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    ADA (Adenosine Deaminase (ADA))

    Andere Bezeichnung

    ADA

    Hintergrund

    Synonyms: Adenosine deaminase

    Tissue Specificity: Found in all tissues, occurs in large amounts in T-lymphocytes (PubMed:20959412). Expressed at the time of weaning in gastrointestinal tissues.

    Background: Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. Primarily, ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.

    Molekulargewicht

    45 kDa

    Gen-ID

    100

    UniProt

    P00813

    Pathways

    Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process
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