Ziege anti-Ratte (Rattus) IgM (Chain mu) Antikörper (DyLight 649) - Preadsorbed

Details zu Produkt Nr. ABIN6699175
Antigen
Epitop
Chain mu
102
82
50
36
19
7
1
1
Reaktivität
Ratte (Rattus)
161
89
45
21
20
16
14
11
11
9
8
8
8
7
7
5
2
2
1
1
Wirt
Ziege
218
105
75
11
11
5
4
2
1
1
1
Klonalität
Polyklonal
Konjugat
DyLight 649
54
47
43
25
23
20
17
9
7
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Optionen
Immunogen

Immunogen: Rat IgM whole molecule

Isotyp IgG
Produktmerkmale

Synonyms: Goat Anti Rat IgM (mu chain) Antibody DyLight 649™ Conjugated, Goat Anti-Rat IgM mu chain Antibody DyLight 649™ Conjugation

Background: Anti-Rat IgM antibody specifically detects rat IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Rat IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology. This Rat IgM Antibody is conjugated to DyLight™649.

Reinigung Preadsorption: Solid phase absorption
Labeling Ratio 3.1
Antigen
Applikations-hinweise

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung RT,4 °C,-20 °C
Informationen zur Lagerung Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Haltbarkeit 12 months
Bilder des Herstellers
Image no. 1 for Goat anti-Rat (Rattus) IgM (Chain mu) antibody (DyLight 649) - Preadsorbed (ABIN6699175) Western Blot of Anti-Rat IgM (mu chain) (GOAT) Antibody. Lane M: 3 μl Molecular Ladde...