EGLN3 Antikörper (AA 151-239)

Produktnummer ABIN669741

Dieses Anti-EGLN3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von EGLN3 in WB, ELISA, IHC (p), IF (cc), IF (p) und IHC (fro). Geeignet für Maus.

Kurzübersicht für EGLN3 Antikörper (AA 151-239) (ABIN669741)

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EGLN3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-EGLN3 Antikörper

Bindungsspezifität: AA 151-239

Kreuzreaktivität: Maus

Homologie: Human,Rat,Dog,Cow,Pig

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from PHD3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu EGLN3

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Andere Bezeichnung: PHD3

Hintergrund:

Synonyms: EGL9 homolog of C. elegans 3, EGLN3, Factor responsive smooth muscle protein, H Prolyl Hydroxylase 3, HP4H3, HPH3, P4H3, Prolyl Hydroxylase Domain Containing Protein 3, SM20, Hypoxia-inducible factor prolyl hydroxylase 3, HIF-PH3, HPH-3.

Background: PHD3 (Egl nine homolog 3, Hypoxia-inducible factor prolyl hydroxylase 3, HIF-prolyl hydroxylase 3, HIF-PH3, HPH-1, Egln3, Prolyl hydroxylase domain-containing protein 3,)Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism. [Catalytic Activity] An HIF alpha chain L-proline + 2-oxoglutarate+ O(2) = An HIF alpha chain trans-4-hydroxy-L-proline + succinate + CO(2). [Subcellular Location] Cytoplasm. Nucleus. Widely expressed at low levels. Expressed athigher levels in heart (cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle) and placenta.

Gen-ID: 112399

UniProt: Q9H6Z9

Pathways: Positive Regulation of Endopeptidase Activity