YDJ1 Antikörper

Produktnummer ABIN6658301

Hersteller Produkt- Nr.: 200-301-f59

Der Maus Monoklonal anti-YDJ1 Antikörper (Klon 2A7-H6) (ABIN6658301) detektiert spezifisch YDJ1 in ELISA, IP und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Saccharomyces cerevisiae.

Kurzübersicht für YDJ1 Antikörper (ABIN6658301)

Target: YDJ1

Reaktivität: Saccharomyces cerevisiae

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Klon: 2A7-H6

Produktdetails

Hersteller Produkt- Nr.: 200-301-f59

Hersteller: Rockland

Verwendungszweck: Hsp40 (YDJ1) Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Hsp40, YDJ1 from yeast based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Hsp40, YDJ1 Antibody was purified by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: Hsp40 (YDJ1) Antibody was produced in mice by repeated immunizations raised against full length protein Hsp40 (YDJ1).

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Western_Blot_Dilution: 1:2000

Kommentare:

Anti-Hsp40, YDJ1 Antibody is tested for use in WB, IP, and ELISA. Expect a band approximately ~40kDa in specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Optional[Buffer]: 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: YDJ1

Hintergrund:

Synonyms: Mitochondrial protein import protein MAS5, Yeast dnaJ protein 1, YDJ1, MAS5, YNL064C, N2418, YNL2418C

Background: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of Hsp70 function. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins. The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1. Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding.

Gene Name: YDJ1

Gen-ID: 855661

NCBI Accession: NP_014335

UniProt: P25491