YDJ1 Antikörper

Produktnummer ABIN6658300

Hersteller Produkt- Nr.: 200-301-e58

Der Maus Monoklonal anti-YDJ1 Antikörper wird verwendet zum Nachweis von YDJ1 in Proben von Saccharomyces cerevisiae. Er wurde validiert für ELISA, IP und WB.

Kurzübersicht für YDJ1 Antikörper (ABIN6658300)

Target: YDJ1

Reaktivität: Saccharomyces cerevisiae

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Klon: 1G10-H8

Produktdetails

Hersteller Produkt- Nr.: 200-301-e58

Hersteller: Rockland

Verwendungszweck: Hsp40 (YDJ1) Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Hsp40 from yeast based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Hsp40, YDJ1 Antibody was purified by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: Hsp40 (YDJ1) Antibody was produced in mice by repeated immunizations raised against full length protein yeast Hsp40 (YDJ1).

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Western_Blot_Dilution: 1:2000

Kommentare:

Anti-Hsp40 Antibody has been tested by western blot and IP and is suitable for ELISA. Expect a band approximately ~40kDa protein corresponding to the molecular mass of Hsp40 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Optional[Buffer]: 50 % (v/v) Glycerol ,0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: YDJ1

Hintergrund:

Synonyms: Mitochondrial protein import protein MAS5, Yeast dnaJ protein 1, YDJ1, MAS5, YNL064C, N2418, YNL2418C

Background: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain. There are two main types of Hsp40, type 1 DNAJ proteins including HDJ2 and yeast YdjI, type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding, type II does not. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins. The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1.

Gene Name: YDJ1

Gen-ID: 855661

NCBI Accession: NP_014335

UniProt: P25491