Tryptophan Hydroxylase 2 Antikörper (pSer19)

Produktnummer ABIN6658283

Hersteller Produkt- Nr.: 612-401-e48

Dieses Anti-Tryptophan Hydroxylase 2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Tryptophan Hydroxylase 2 in WB. Geeignet für Ratte.

Kurzübersicht für Tryptophan Hydroxylase 2 Antikörper (pSer19) (ABIN6658283)

Target: Tryptophan Hydroxylase 2 (TPH2)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tryptophan Hydroxylase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Tryptophan Hydroxylase 2 Antikörper

Bindungsspezifität: pSer19

Hersteller Produkt- Nr.: 612-401-e48

Hersteller: Rockland

Verwendungszweck: Tryptophan Hydroxylase 2 phospho S19 Antibody

Kreuzreaktivität (Details): Anti-Tryptophan Hydroxylase 2 is directed against rat Tryptophan Hydroxylase phosphorylated at Ser19.

Aufreinigung: Tryptophan Hydroxylase 2 pS19 antibodies are prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-Tryptophan Hydroxylase 2 pS19 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser19 of Tryptophan Hydroxylase.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optional[Neutralization_Dilution]: 1:1000

Kommentare:

Anti-Trytophan Hydroxylase2 pS19 (Rabbit) antibody is tested for use in Western Blotting. Expect a band of approximately 55 kDa in size corresponding to tryptophan hydroxylase protein phosphorylated at Ser 19 in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Tryptophan Hydroxylase 2

Target: Tryptophan Hydroxylase 2 (TPH2)

Andere Bezeichnung: Tryptophan Hydroxylase 2

Hintergrund:

Synonyms: Tryptophan 5-hydroxylase 2, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2, TPH

Background: Tryptophan Hydroxylase 2 pS19 antibody detects TPH2 protein. Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2. Anti-TPH2pS19 antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.

Gene Name: TPH2

Gen-ID: 317675

UniProt: Q8CGU9