YWHAB Antikörper (pSer58)

Produktnummer ABIN6658145

Hersteller Produkt- Nr.: 600-401-d02

Produktdetails anti-YWHAB Antikörper

Target: YWHAB (14-3-3 alpha + beta (YWHAB))

Bindungsspezifität: pSer58

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser YWHAB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 600-401-d02

Hersteller: Rockland

Verwendungszweck: 14-3-3 phospho S58 Antibody

Kreuzreaktivität (Details): Anti-14-3-3 pS58 antibody is directed against rat 14-3-3 protein phosphorylated at S58.

Aufreinigung: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-14-3-3 pS58 antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: 1:500

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-14-3-3 pS58 antibody is tested for use in ELISA, Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa in size corresponding to the 14-3-3 proteins phosphorylated at Ser58 in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu YWHAB

Target: YWHAB (14-3-3 alpha + beta (YWHAB))

Andere Bezeichnung: Ywhab (YWHAB Produkte)

Hintergrund:

Synonyms: 14-3-3 protein beta/alpha, Prepronerve growth factor RNH-1, Protein kinase C inhibitor protein 1

Background: 14-3-3 pS58 antibody detects 14-3-3 protein phosphorylated at S58. 14-3-3 is a member of a family of highly conserved proteins that appear to have multiple roles in cell signaling. 14-3-3 is abundantly expressed in the brain and has been detected in the cerebrospinal fluid of patients with different neurological disorders. 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms. 14-3-3 affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation. Anti-14-3-3 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: YWHAB

Gen-ID: 56011

NCBI Accession: NP_062250

UniProt: P35213

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Myometrial Relaxation and Contraction, Maintenance of Protein Location