CGRP Antikörper

Produktnummer ABIN6658144

Hersteller Produkt- Nr.: 200-301-d15

Produktdetails anti-CGRP Antikörper

Target: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CGRP Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Hersteller Produkt- Nr.: 200-301-d15

Hersteller: Rockland

Verwendungszweck: Calcitonin Gene Related Peptide (CGRP) Antibody

Kreuzreaktivität (Details): Anti-CGRP Monoclonal Antibody is directed against rat CGRP protein.

Aufreinigung: The antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Immunogen: Anti-CGRP Monoclonal Antibody was produced by repeated immunizations with purified rat alpha-CGRP.

Klon: 4901

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: 1:50-1:100

ELISA_Dilution: 1:5,000

Immunohistochemistry_Dilution: 1:50-1:100

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: not recommended

Other: ICC: 1:50-1:100

Kommentare:

Suggested Applications: IF, IHC
Anti-Calcitonin Gene Related Peptide antibody has been tested by ELISA and ICC. This product is not recommended for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Suggested positive controls are dorsal root ganglia, pancreas and gut.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-CGRP Antikörper (ABIN6658144)

Morelli, Castaldi, Brown, Streich, Websdale, Taberner, Cerreti, Barenghi, Blum, Sawitzke, Frank, Steffens, Doleschall, Serrao, Ferrarini, Lechner, Prevedel, Heppenstall: "Identification of a population of peripheral sensory neurons that regulates blood pressure." in: Cell reports, Vol. 35, Issue 9, pp. 109191, (2022) (PubMed).

Webber, Clancy, Zhou, Yraola, Homma, García-Añoveros: "Axodendritic versus axosomatic cochlear efferent termination is determined by afferent type in a hierarchical logic of circuit formation." in: Science advances, Vol. 7, Issue 4, (2021) (PubMed).

Dhandapani, Arokiaraj, Taberner, Pacifico, Raja, Nocchi, Portulano, Franciosa, Maffei, Hussain, de Castro Reis, Reymond, Perlas, Garcovich, Barth, Johnsson, Lechner, Heppenstall: "Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons." in: Nature communications, Vol. 9, Issue 1, pp. 1640, (2019) (PubMed).

Details zu CGRP

Target: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Andere Bezeichnung: Calcitonin Gene Related Peptide (CGRP) (CALCA Produkte)

Hintergrund:

Synonyms: Alpha-type CGRP, Calcitonin gene-related peptide I

Background: CGRP antibody recognizes Calcitonin Gene Related Peptide or CGRP which is a 37 amino acid peptide and is the most potent endogenous vasodilator currently known. CGRP is primarily produced in nervous tissue, however, its receptors are expressed throughout the body. CGRP is also strongly implicated in the vasodilatory effect of endogenous cannabinoid anandamide in the brain. This effect was found to be antagonized by capsazepine. CGRP is also currently a major target of research in regards to factors effecting the onset of migraine headaches. CGRP antibodies have been used successfully in immunoneutralization. Anti-CGRP antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: CALCA

Gen-ID: 24241

NCBI Accession: NP_001029128

UniProt: P01256

Pathways: Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour