INA Antikörper

Produktnummer ABIN6658126

Hersteller Produkt- Nr.: 200-901-d04

Der Huhn Polyklonal Anti-INA-Antikörper wurde für WB, IHC, ELISA, FM und MA validiert. Er ist geeignet, INA in Proben von Ratte zu detektieren.

Kurzübersicht für INA Antikörper (ABIN6658126)

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Reaktivität: Ratte

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser INA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM), Multiplex Assay (MA)

Produktdetails anti-INA Antikörper

Hersteller Produkt- Nr.: 200-901-d04

Hersteller: Rockland

Verwendungszweck: Alpha Internexin Antibody

Kreuzreaktivität (Details): Anti-Alpha Internexin Antibody is directed against rat Alpha Internexin proteins.

Aufreinigung: The antibody was purified from chicken eggs as an IgY fraction.

Immunogen: Anti-Alpha Internexin Antibody was produced by repeated immunizations with recombinant rat alpha internexin expressed in E. coli.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:50,000

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:5000

Kommentare:

Anti-Alpha Internexin antibody is tested for use in Western Blotting and ICC, and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 66 kDa in size corresponding to the alpha internexin protein in Western blots in the appropriate cell lysate or extract. Minor bands at ~150k are probably covalent dimers and bands at ~50k represent alpha-internexin breakdown products.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu INA

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Andere Bezeichnung: Alpha Internexin

Hintergrund:

Synonyms: Alpha INX, internexin, NF-66, NEF5

Background: Anti-Alpha Internexin antibody recognizes alpha-internexin which is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits. Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder. The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals. Anti-Alpha Internexin antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: INA

Gen-ID: 24503

UniProt: P23565