SH3GL1 Antikörper (C-Term)

Produktnummer ABIN6658121

Hersteller Produkt- Nr.: 200-301-g41

Dieses Anti-SH3GL1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von SH3GL1 in WB, IHC, IP und FM. Geeignet für Ratte.

Kurzübersicht für SH3GL1 Antikörper (C-Term) (ABIN6658121)

Target: SH3GL1 (SH3-Domain GRB2-Like 1 (SH3GL1))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SH3GL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Klon: S51-1

Produktdetails anti-SH3GL1 Antikörper

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 200-301-g41

Hersteller: Rockland

Verwendungszweck: SH3GL1 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with SH3GL1 from Mouse, human, and rat based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-SH3GL1 Antibody was purified by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: SH3GL1 Antibody was produced in mice by repeated immunizations raised against a synthetic peptide corresponding to the near c-terminal region of rat SH3GL1.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: 0.1-1.0 μg/mL (Perox), 1-10 μg/mL (IF)

IF_Microscopy_Dilution: 1.0-10 μg/mL

Western_Blot_Dilution: 1 μg/mL

Kommentare:

Anti-SH3GL1 Antibody is suitable for use in WB, IHC and IP. Expect a band approximately ~43kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu SH3GL1

Target: SH3GL1 (SH3-Domain GRB2-Like 1 (SH3GL1))

Andere Bezeichnung: SH3GL1

Hintergrund:

Synonyms: SH3 domain GRB2 like 1 protein, CNSA1, SH3P8, EEN, Endophilin-A2, EEN fusion partner of MLL, Endophilin-2, Extra eleven-nineteen leukemia fusion gene protein, SH3 domain protein 2B

Background: Endophilin-A2 is a protein that in humans is encoded by the SH3GL1 gene. Endophilin-A2 is an SH3 domaincontaining protein that is involved in the clathrin-mediated endocytosis and recycling of synaptic vesicles. Its SH3 domain lies at the C-terminus of the protein, and can bind proline-rich sequences on other endocytotic proteins. It also binds the C-terminus of the voltage-gated calcium channel in a calcium-dependent manner, which serves to enrich the endocytotic machinery at nerve termincals.

Gene Name: SH3GL1

Gen-ID: 6455

NCBI Accession: NP_003016

UniProt: Q99961