SHANK3 Antikörper (Internal Region)

Produktnummer ABIN6658092

Hersteller Produkt- Nr.: 200-301-g45

Dieses Maus Monoklonal-Antikörper erkennt spezifisch SHANK3 in WB, IHC, IP, IF und FM. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für SHANK3 Antikörper (Internal Region) (ABIN6658092)

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SHANK3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Klon: S69

Produktdetails anti-SHANK3 Antikörper

Bindungsspezifität: Internal Region

Hersteller Produkt- Nr.: 200-301-g45

Hersteller: Rockland

Verwendungszweck: SHANK3 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with SHANK3 from human, mouse, and rat based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-SHANK3 Antibody was purified by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: SHANK3 Antibody was produced in mice by repeated immunizations raised against a synthetic peptide corresponding to an internal region of rat Shank3.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: 0.1-1.0 μg/mL

IF_Microscopy_Dilution: 1.0-10 μg/mL

Western_Blot_Dilution: 1-10 μg/mL

Kommentare:

Anti-SHANK3 Antibody is tested for use in WB, IHC, IF, and IP. Expect a band approximately ~190kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu SHANK3

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Andere Bezeichnung: SHANK3

Hintergrund:

Synonyms: Shank3, Prosap2, SH3 and multiple ankyrin repeat domains protein 3, Proline-rich synapse-associated protein 2

Background: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are also crucial in receptor tyrosine kinase signaling, specifically, Shank3 can mediate Erk-MAPK and P13K signaling which is crucial for tubule formation. Shank3 is also one of the latest genes to be associated with autism. A mutation of a single copy of Shank3 on chromosome 22q13 can result in language and/or social communication disorders.

Gene Name: Shank3

Gen-ID: 59312

NCBI Accession: NP_067708

UniProt: Q9JLU4

Pathways: Synaptic Membrane, Tube Formation, Regulation of long-term Neuronal Synaptic Plasticity