MBP Antikörper

Produktnummer ABIN6658054

Produktdetails anti-MBP Antikörper

Target: MBP (Myelin Basic Protein (MBP))

Reaktivität: Ratte

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser MBP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Fluorescence Microscopy (FM)

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte (Rattus)

Kreuzreaktivität (Details): Cross reactivity with MBP from other mammalian tissues is expected.

Aufreinigung: Anti-MBP antibody is directed against Myelin basic protein.The antibody is a total IgY fraction. The antibody has been directly tested for reactivity in bovine, human, mouse and rat tissues.

Immunogen:

Immunogen: Anti-Myelin basic protein Antibody was produced in chicken by repeated immunizations with three peptide sequences conserved in higher vertebrate MBP proteins.

Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Myelin Basic Protein antibody is suitable for use in Western Blotting and IF. Expect a bands of approximately 18 kDa and 22 kDa in size corresponding to MBP proteins in Western blots of rat brain homogenate. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:5000

IF Microscopy Dilution: 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.20.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Details zu MBP

Target: MBP (Myelin Basic Protein (MBP))

Abstract: MBP Produkte

Synonyme: C76307 antikoerper, Hmbpr antikoerper, R75289 antikoerper, golli-mbp antikoerper, mld antikoerper, shi antikoerper, Mbps antikoerper, cb274 antikoerper, fj33b11 antikoerper, mbp antikoerper, wu:fj33b11 antikoerper, wu:fq15b02 antikoerper, zgc:136630 antikoerper, MGC115135 antikoerper, myelin basic protein antikoerper, myelin basic protein a antikoerper, myelin basic protein L homeolog antikoerper, MBP antikoerper, Mbp antikoerper, mbpa antikoerper, mbp antikoerper, mbp.L antikoerper

Hintergrund:

Synonyms: Myelin A1 protein, Myelin membrane encephalitogenic protein, Myelin basic protein, MBP, Myelin basic protein S, MBP S antibody

Background: Myelin Basic Protein antibody detects MBP. MBP is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago. The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells. In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22 kDa and another at about 18 kDa. Anti-MBP antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Signal Transduction research.

Gene Name: MDV1

Gen-ID: 24547

NCBI Accession: NP_001020465

UniProt: P02688