MBP Antikörper

Produktnummer ABIN6658054

Hersteller Produkt- Nr.: 200-901-d81

Der Huhn Polyklonal Anti-MBP-Antikörper wurde für WB, IF und FM validiert. Er ist geeignet, MBP in Proben von Ratte zu detektieren.

Kurzübersicht für MBP Antikörper (ABIN6658054)

Target: MBP (Myelin Basic Protein (MBP))

Reaktivität: Ratte

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser MBP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Produktdetails anti-MBP Antikörper

Hersteller Produkt- Nr.: 200-901-d81

Hersteller: Rockland

Verwendungszweck: Myelin Basic Protein (MBP) Antibody

Kreuzreaktivität (Details): Anti-MBP antibody is directed against Myelin basic protein.

Aufreinigung: The antibody is a total IgY fraction.

Immunogen: Anti-Myelin basic protein Antibody was produced in chicken by repeated immunizations with three peptide sequences conserved in higher vertebrate MBP proteins.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:5000

Kommentare:

Anti-Myelin Basic Protein antibody is tested for use in Western Blotting and IF. Expect a bands of approximately 18 kDa and 22 kDa in size corresponding to MBP proteins in Western blots of rat brain homogenate. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu MBP

Target: MBP (Myelin Basic Protein (MBP))

Hintergrund:

Synonyms: Myelin A1 protein, Myelin membrane encephalitogenic protein, Myelin basic protein, MBP, Myelin basic protein S, MBP S antibody

Background: Myelin Basic Protein antibody detects MBP. MBP is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago. The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells. In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22 kDa and another at about 18 kDa. Anti-MBP antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Signal Transduction research.

Gene Name: MDV1

Gen-ID: 24547

NCBI Accession: NP_001020465

UniProt: P02688