NEFM Antikörper (C-Term)

Produktnummer ABIN6658051

Produktdetails anti-NEFM Antikörper

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Bindungsspezifität: C-Term

Reaktivität: Ratte

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser NEFM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Fluorescence Microscopy (FM)

Kreuzreaktivität: Huhn, Rind (Kuh), Human, Maus, Ratte (Rattus)

Kreuzreaktivität (Details): Cross reactivity with NF-M from other species has not been determined.

Aufreinigung: Anti-NF-M antibody is directed against NF-M protein. The antibody is a total IgY fraction. Expect reactivity with the following species based on sequence homology: chicken, human, mouse.

Immunogen:

Immunogen: Anti-NF-M Monoclonal Antibody was produced by repeated immunizations with recombinant C-terminus part of NF-M expressed in E. coli.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti- Neurofilament M antibody is suitable for use in Western Blotting and IF. Specific . Expect a band of approximately 145 kDa in size corresponding to NF-M proteins in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:5000

IF Microscopy Dilution: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.20.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Referenzen für anti-NEFM Antikörper (ABIN6658051)

Martineau, Di Polo, Vande Velde, Robitaille: "Dynamic neuromuscular remodeling precedes motor-unit loss in a mouse model of ALS." in: eLife, Vol. 7, (2019) (PubMed).

Darabid, Arbour, Robitaille: "Glial cells decipher synaptic competition at the mammalian neuromuscular junction." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 4, pp. 1297-313, (2013) (PubMed).

Details zu NEFM

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Andere Bezeichnung: Neurofilament M (NEFM Produkte)

Synonyme: NEF3 antikoerper, NF-M antikoerper, NFM antikoerper, Nef3 antikoerper, Nfm antikoerper, NF160 antikoerper, NF165 antikoerper, nef-m antikoerper, nef3 antikoerper, nefm antikoerper, nf-m antikoerper, zgc:112359 antikoerper, nefm-a antikoerper, nefm-b antikoerper, neurofilament medium antikoerper, neurofilament triplet M protein antikoerper, neurofilament, medium polypeptide antikoerper, neurofilament medium polypeptide antikoerper, neurofilament medium L homeolog antikoerper, neurofilament, medium polypeptide a antikoerper, neurofilament medium S homeolog antikoerper, NEFM antikoerper, Nefm antikoerper, TVAG_582010 antikoerper, TVAG_379630 antikoerper, nefm.L antikoerper, nefma antikoerper, nefm.S antikoerper

Hintergrund:

Synonyms: chicken anti-Neurofilament M antibody, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein

Background: NF-M antibody detects Neurofilament M protein. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease. Neurofilament M Antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.

Gene Name: NEFM

Gen-ID: 24588

UniProt: P12839

Pathways: Brown Fat Cell Differentiation