NOX1 Antikörper (Internal Region)

Produktnummer ABIN6658038

Hersteller Produkt- Nr.: 600-401-r15

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NOX1 in WB und IHC. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für NOX1 Antikörper (Internal Region) (ABIN6658038)

Target: NOX1 (NADPH Oxidase 1 (NOX1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-NOX1 Antikörper

Bindungsspezifität: Internal Region

Hersteller Produkt- Nr.: 600-401-r15

Hersteller: Rockland

Verwendungszweck: Nox1 Antibody

Kreuzreaktivität (Details): Anti-NOX1 antibody is directed against rat NOX1 protein.

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: NOX1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of rat NOX1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 0.5 μg/mL

Kommentare:

Anti-NOX1 is tested in Immunohistochemistry -P and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~65.2 kDa corresponding to the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu NOX1

Target: NOX1 (NADPH Oxidase 1 (NOX1))

Andere Bezeichnung: NOX1

Hintergrund:

Synonyms: Mitogenic oxidase 1,

Background: NOX1(NADPH OXIDASE 1), also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. The NOX1 gene is mapped to Xq22.1. NOX1 was expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58 % identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of MOX1 in NIH 3T3 cells increased superoxide generation and cell growth. Cells expressing MOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50 % survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion. This antibody is suitable for researchers interested in epigenetic antibodies and cancer research.

Gene Name: NOX1

Gen-ID: 114243

NCBI Accession: NP_446135

UniProt: Q9WV87

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Proton Transport