DAB1 Antikörper (AA 400-555)

Produktnummer ABIN6658036

Hersteller Produkt- Nr.: 100-401-225s

Der Kaninchen Polyklonal Anti-DAB1-Antikörper wurde für WB, ELISA, IHC, IP und FM validiert. Er ist geeignet, DAB1 in Proben von Maus zu detektieren. Es sind 9+ Publikationen verfügbar.

Kurzübersicht für DAB1 Antikörper (AA 400-555) (ABIN6658036)

Target: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DAB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Produktdetails anti-DAB1 Antikörper

Bindungsspezifität: AA 400-555

Hersteller Produkt- Nr.: 100-401-225s

Hersteller: Rockland

Verwendungszweck: Dab1 Antibody

Kreuzreaktivität (Details): This antibody is directed against Dab1 from mouse.

Aufreinigung: Dab-1 whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative.

Sterilität: Sterile filtered

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of murine Dab1 at amino acids 400-555.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: 1 μL per 500 μg

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:5,000

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1:5,000

Other: User Optimized

Kommentare:

Suggested Applications: Biochemical Assay, IF, Multiplex
Anti-Dab1 antibody has been tested by western blot and immunohistochemistry and is suitable for the detection of Dab1 by immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user.  Expect a band at 80 kDa corresponding to Dab1 in the appropriate tissue extract or cell lysate.  For western blotting block the blot using 5% BLOTTO for 1 h at room temperature and followed by incubation with the primary antibody diluted in 1% BLOTTO in TTBS for 1 h at room temperature.  For immunoprecipitation use 1 μl of antiserum per 500 μg of brain lysate.  Perform immunoprecipitation at 4°C for 2 h.  For immunoprecipitation buffer lysates with 50 mM Tris-Cl, pH 7.4, supplemented with 150 mM sodium chloride, 1% (v/v) NP-40, 10 μg/ml aprotinin and 10 μg/ml leupeptin.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-DAB1 Antikörper (ABIN6658036)

Wang, Lei, Wang, Yu, He, Zhao, Hu, Xu, Jin, Gu, Guo, Yang, Gao, Wang: "The ZSWIM8 ubiquitin ligase regulates neurodevelopment by guarding the protein quality of intrinsically disordered Dab1." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 33, Issue 7, pp. 3866-3881, (2023) (PubMed).

Reyes, Hino, Canales, Dickson, La Torre, Simó: "The E3 Ubiquitin Ligase CRL5 Regulates Dentate Gyrus Morphogenesis, Adult Neurogenesis, and Animal Behavior." in: Frontiers in neuroscience, Vol. 16, pp. 908719, (2022) (PubMed).

Meseke, Neumüller, Brunne, Li, Anstötz, Pohlkamp, Rogalla, Herz, Rune, Bender: "Distal Dendritic Enrichment of HCN1 Channels in Hippocampal CA1 Is Promoted by Estrogen, but Does Not Require Reelin." in: eNeuro, Vol. 5, Issue 5, (2019) (PubMed).

Fairchild, Hino, Han, Miltner, Peinado Allina, Brown, Burns, La Torre, Simó: "RBX2 maintains final retinal cell position in a DAB1-dependent and -independent fashion." in: Development (Cambridge, England), Vol. 145, Issue 3, (2018) (PubMed).

O'Brien, Hirano, Buttermore, Bhat, Peles: "Localization of the paranodal protein Caspr in the mammalian retina." in: Molecular vision, Vol. 16, pp. 1854-63, (2011) (PubMed).

de la Maza, Olivares, Hirsch, Sierralta, Gattás, Barrera, Bunout, Leiva, Fernández: "Weight increase and overweight are associated with DNA oxidative damage in skeletal muscle." in: Clinical nutrition (Edinburgh, Scotland), Vol. 25, Issue 6, pp. 968-76, (2007) (PubMed).

Katyal, Gao, Monckton, Glubrecht, Godbout: "Hierarchical disabled-1 tyrosine phosphorylation in Src family kinase activation and neurite formation." in: Journal of molecular biology, Vol. 368, Issue 2, pp. 349-64, (2007) (PubMed).

Hanzlicek, Peachey, Grimm, Hagstrom, Ball: "Probing inner retinal circuits in the rod pathway: a comparison of c-fos activation in mutant mice." in: NULL, Vol. 21, Issue 6, pp. 873-81, (2005) (PubMed).

Bramblett, Pennesi, Wu, Tsai: "The transcription factor Bhlhb4 is required for rod bipolar cell maturation." in: Neuron, Vol. 43, Issue 6, pp. 779-93, (2004) (PubMed).

Details zu DAB1

Target: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Andere Bezeichnung: Dab1

Hintergrund:

Synonyms: rabbit anti-Dab 1 antibody, Disabled homolog 1 antibody, Disabled homolog 1 Drosophila antibody, Scm antibody, Scr antibody, Scrambler antibody, Yot antibody, Yotari antibody

Background: Anti-Dab1 Antibody recognizes Dab1 that is a phosphoprotein encoded by the mouse gene dab and is related to the Drosophila gene 'disabled'. Mutations in the mouse dab gene may result in the 'scrambler' and 'yotari' phenotypes. Dab1 binds to non-receptor tyrosine kinases and plays an important role in brain development. Dab1 is expressed in neuronal populations exposed to reelin, and it functions as a signaling molecule that regulates cell positioning in the developing brain. Cloning of human Dab1 and sequence determinations suggest a 96 % identity to the mouse sequence. Dab1 binds to cytoplasmic regions of very low density lipoprotein receptors (VLDLR), apolipoprotein E receptor-2 (ApoER2) and the Amyloid Precursor Protein (APP) family of proteins. Dab1 accumulates in ectopic neurons from mice lacking Reelin or both VLDLR and ApoER2. In humans, Dab1 has been mapped to lp32-p312. This region shows homology of synteny with the segment of mouse chromosome 4 containing Dab1.

Gene Name: DAB1

Gen-ID: 13131

NCBI Accession: NP_034144

UniProt: P97318