TNNI3 Antikörper (pSer150)

Produktnummer ABIN6658032

Hersteller Produkt- Nr.: 600-401-x33

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch TNNI3 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für TNNI3 Antikörper (pSer150) (ABIN6658032)

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNNI3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-TNNI3 Antikörper

Bindungsspezifität: pSer150

Hersteller Produkt- Nr.: 600-401-x33

Hersteller: Rockland

Verwendungszweck: Troponin I (cardiac) Phospho S150 Antibody

Kreuzreaktivität (Details): Anti-Phospho-Ser150 Troponin (cardiac) Antibody is directed against Troponin protein phosphorylated at S150.

Aufreinigung: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen: Anti-Phospho-Ser150 Troponin (cardiac) Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 pf cardiac troponin I conjugated to KLH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Phospho-Ser150 Troponin (cardiac) Antibody has been tested in Western Blots and is specific for the ~25 kDa cardiac troponin I protein phosphorylated at Ser 150. Immunolabeling is greatly decreased with lambda-phosphatase treatment. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Haltbarkeit: 12 months

Details zu TNNI3

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Andere Bezeichnung: Troponin I (cardiac)

Hintergrund:

Synonyms: Troponin I cardiac muscle, Cardiac troponin I

Background: Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 and is phosphorylated by AMPK at Ser23 and Ser150. Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser150 over Ser23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI. Troponin I pS150 Antibody is ideal for researchers interested in cancer research.

Gene Name: TNNI3

Gen-ID: 21954

NCBI Accession: NP_033432

UniProt: P48787