TNNI3 Antikörper (pSer43)

Produktnummer ABIN6658030

Hersteller Produkt- Nr.: 600-401-x34

Der Kaninchen Polyklonal Anti-TNNI3-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, TNNI3 in Proben von Maus zu detektieren.

Kurzübersicht für TNNI3 Antikörper (pSer43) (ABIN6658030)

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNNI3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-TNNI3 Antikörper

Bindungsspezifität: pSer43

Hersteller Produkt- Nr.: 600-401-x34

Hersteller: Rockland

Verwendungszweck: Troponin I (Cardiac) Phospho S43 Antibody

Kreuzreaktivität (Details): Anti-Phospho-Ser43 Troponin (cardiac) Antibody is directed against Troponin protein phosphorylated at S43.

Aufreinigung: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen: Anti-Phospho-Ser43 Troponin (cardiac) Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser43 of mouse cardiac troponin I conjugated to KLH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Phospho-Ser43 Troponin (cardiac) Antibody has been tested by Western Blots and is specific for the ~25 kDa cardiac troponin I protein phosphorylated at Ser 43. Immunolabeling is greatly decreased with lambda-phosphatase treatment. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Haltbarkeit: 12 months

Details zu TNNI3

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Andere Bezeichnung: Troponin I (Cardiac)

Hintergrund:

Synonyms: Troponin I cardiac muscle, Cardiac troponin I

Background: Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. Protein kinase C-mediated phosphorylation of cardiac myofilament proteins has been shown to depress the actomyosin interaction and may be important during heart failure. Studies indicate that phosphorylation of Ser43 and Ser45 of cardiac troponin I plays a substantial role in the PKC-mediated depression. Troponin I pS43 Antibody is ideal for researchers interested in cancer research.

Gene Name: TNNI3

Gen-ID: 21954

NCBI Accession: NP_033432

UniProt: P48787