NrCAM Antikörper (AA 30-845) (PerCP)

Produktnummer ABIN6658004

Hersteller Produkt- Nr.: 200-365-w74

Dieses Anti-NrCAM-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von NrCAM in WB, ELISA und IHC. Geeignet für Maus.

Kurzübersicht für NrCAM Antikörper (AA 30-845) (PerCP) (ABIN6658004)

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NrCAM Antikörper ist konjugiert mit PerCP

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Klon: S364-51

Produktdetails anti-NrCAM Antikörper (PerCP)

Bindungsspezifität: AA 30-845

Hersteller Produkt- Nr.: 200-365-w74

Hersteller: Rockland

Verwendungszweck: NrCAM Antibody PerCP

Kreuzreaktivität (Details): BLAST analysis suggests that it is 96 % identical to rat, 91 % identical to human and ~50 % identical to Neurofascin.

Aufreinigung: Anti-NrCAM Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: Anti-NrCAM Antibody was produced by repeated immunization of mice with a fusion protein containing amino acids 30-845 of mouse NrCAM.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-NrCAM PerCP Conjugated Antibody is suitable for Western Blots and Immunocytochemistry. Expect a band approximately ~160kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu NrCAM

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Andere Bezeichnung: NrCAM

Hintergrund:

Synonyms: Neuronal cell adhesion molecule, Bravo, hBravo, KIAA0343, MGC138845, MGC138846, Neuronal surface protein Bravo, Ng CAM related, Ng-CAM-related, NgCAM related cell adhesion molecule, NgCAM-related cell adhesion molecule, Nr CAM, Nr-CAM

Background: Neuronal cell adhesion molecule (NrCAM) is a cell surface protein of the immunoglobulin (Ig) superfamily. NrCAM (also known as Bravo) contains six Ig domains, five fibronectin repeats, a transmembrane region and an intracellular domain. NrCAM is expressed in brain, spinal cord, peripheral nervous system and pancreas. In the spinal cord, NrCAM acts as a ligand for axonin-1 to guide commissural axons across the floor plate. NrCAM also acts as a ligand for F3 to control actin-dependent growth cone motility. NrCAM interacts with neurofascin and may facilitate the clustering of the cystoskeletal protein ankyrin G and the voltage-dependent sodium channel proteins at the node of Ranvier. NrCAM expression may play a role in the severity of certain types of tumors. NrCAM is overexpressed in high-grade astrocytomas, gliomas and glioblastoma tumor tissues. Anti-NrCam is ideal for research in Cancer and Neuroscience. In the pancreas, NrCAM expression is upregulated in intraductal hyperplasia. Antisense NrCAM reduces the tumorigenic properties of human glioblastoma cells in vitro and slowed tumor growth in vivo. The gene encoding human NrCAM maps to chromosome 7q31.1-q31.2.

Gene Name: NrCAM

Gen-ID: 319504

NCBI Accession: NP_001139503

UniProt: Q810U4

Pathways: Regulation of Cell Size