L-Selectin Antikörper (APC)

Produktnummer ABIN6657789

Hersteller Produkt- Nr.: 200-526-n87

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch L-Selectin in FACS, IHC und IP. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für L-Selectin Antikörper (APC) (ABIN6657789)

Target: L-Selectin (SELL) (Selectin L (SELL))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser L-Selectin Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Klon: MEL-14

Produktdetails anti-L-Selectin Antikörper (APC)

Hersteller Produkt- Nr.: 200-526-n87

Hersteller: Rockland

Verwendungszweck: CD62L Allophycocyanin Antibody

Kreuzreaktivität (Details): Cross reactivity with CD62L from other sources has not been tested.

Aufreinigung: Allophycocyanin conjugated CD62L Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD62L.

Sterilität: Sterile filtered

Immunogen: Anti-CD62L Antibody (Monoclonal) was produced by repeated immunizations with CD62L antigen.

Isotyp: IgG2a kappa

Labeling Ratio: 1-2

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Kommentare:

Anti-CD62L is tested for FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Haltbarkeit: 6 months

Details zu L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Andere Bezeichnung: CD62L

Hintergrund:

Synonyms: L-selectin CD62 antigen-like family member L Leukocyte adhesion molecule 1 LAM-1 Leukocyte-endothelial cell adhesion molecule 1 LECAM1 Lymph node homing receptor Lymphocyte antigen 22 Ly-22 Lymphocyte surface MEL-14 antigen CD62L Sell Lnhr, Ly-22, Ly22

Background: Anti-CD62L antibody is specific for mouse CD62L, also known as L-Selectin, a cell adhesion molecule which facilitates lymphocyte rolling on activated vascular endothelium and homing to high endothelial venules (HEV) as immune cells transmigrate from blood into peripheral tissues. L-Selectin is a member of a family of Selectin molecules which act together with the integrin family of adhesion molecules to mediate leukocyte-endothelial interactions. L-Selectin is characteristically expressed by neutrophils, and is also found on B cells, monocytes, granulocytes, and at varying levels on naive, effector and memory T cells. It is rapidly shed upon cell activation, releasing into the circulation a soluble form whose biological role is of particular interest in cancer biology research.The MEL-14 antibody may be used as a phenotypic marker for CD62L expression on a variety of immune cell types. Please note that CD62L (L-Selectin) itself is also referred to as MEL-14 in the literature.

Gene Name: Sell

Gen-ID: 20343

NCBI Accession: NP_035476

UniProt: P18337