BAT3 Antikörper (C-Term)

Produktnummer ABIN6657746

Produktdetails anti-BAT3 Antikörper

Target: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Bindungsspezifität: C-Term

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BAT3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Aufreinigung: This product was prepared from monospecific antiserum by addition of Sodium azide to 0.01%. The antibody detects endogenous Scythe/BAT3 in cell lysates.

Immunogen:

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal portion of mouse Scythe/BAT3.

Immunogen Type: Peptide

Anwendungsinformationen

Applikationshinweise:

Application Note: This polyclonal antibody has been tested for use in western blotting and is specific for mouse Scythe/Bat3 and its isoforms. Specific conditions for reactivity should be optimized by the end user. Expect multiple bands, with the predominant at approximately 200 kDa in size corresponding to Scythe/BAT3 by western blotting in the appropriate cell lysate or extract. In western blot analysis of endogenous protein, multiple bands of Scythe/BAT3 may appear corresponding to variable amount of splice isoforms present.

ELISA Dilution: User Optimized

Western Blot Dilution: 1:5,000 to 1:8000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Details zu BAT3

Target: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Andere Bezeichnung: Scythe/Bat3 (BAT3 Produkte)

Synonyme: ARABIDOPSIS THALIANA BCL-2-ASSOCIATED ATHANOGENE 6 antikoerper, ATBAG6 antikoerper, BCL-2-associated athanogene 6 antikoerper, T3F17.11 antikoerper, 2410045D21Rik antikoerper, AA408914 antikoerper, BAG-6 antikoerper, Bat3 antikoerper, D17H6S52E antikoerper, G3 antikoerper, Scythe antikoerper, BAT3 antikoerper, D6S52E antikoerper, BCL-2-associated athanogene 6 antikoerper, BCL2-associated athanogene 6 antikoerper, BCL2 associated athanogene 6 antikoerper, BAG6 antikoerper, Bag6 antikoerper

Hintergrund:

Synonyms: rabbit anti-Bat3 Antibody, rabbit anti-Scythe Antibody, Human Leukocyte Antigen-B-Associated Transcript 3, HLA-B-associated transcript 3, BAT3, Large proline-rich protein BAT3

Background: Scythe protein (also known as BAT3 and HLA-B-associated transcript 3) is an apoptotic regulator that is highly conserved in eukaryotes and contains a ubiquitin-like domain near its N-terminus. It binds Reaper, a potent apoptotic inducer, as well as Hid and Grim. Scythe and Reaper are thought to signal apoptosis, in part through regulating the folding and activity of apoptotic signaling molecules. Scythe regulates apoptosis-inducing factor stability during endoplasmic reticulum stress-induced apoptosis. It interacts with transforming growth factor-beta (TGF-beta) receptors and enhances TGF-beta1-induced type I collagen expression in mesangial cells. With SET1A, Scythe forms a complex with CTCFL/BORIS to modulate H3K4 histone dimethylation and gene expression. Scythe is essential for p300-mediated acetylation of p53. Scythe/BAT3 is identified as a critical regulator of Hsp70-2 in spermatogenesis, thereby providing a possible molecular target in idiopathic male infertility. The human Scythe/BAT3 ortholog in rodents is predominantly and developmentally expressed in testis.

Gene Name: BAT3

Gen-ID: 224727, 33147082

UniProt: Q9Z1R2