HSF1 Antikörper (AA 425-439) (FITC)

Produktnummer ABIN6657636

Hersteller Produkt- Nr.: 200-502-w89

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch HSF1 in WB, ELISA, IP und GS. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für HSF1 Antikörper (AA 425-439) (FITC) (ABIN6657636)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser HSF1 Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Gel Shift (GS)

Klon: 4B4

Produktdetails anti-HSF1 Antikörper (FITC)

Bindungsspezifität: AA 425-439

Hersteller Produkt- Nr.: 200-502-w89

Hersteller: Rockland

Verwendungszweck: HSF1 Antibody Fluorescein

Aufreinigung: Anti-HSF1 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: Anti-HSF1 Antibody was produced by repeated immunization of rats with a purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:10,000

Gel_Shift_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-HSF1 FITC Conjugated Antibody is suitable for Western Blots, Immunoprecipitation, ELISA, Gel Mobility Shift Assay and Immunocytochemistry. Expect a band approximately ~85kDa protein in unstressed cell lysates, and a 95 kDa protein in heat shocked cell lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Andere Bezeichnung: HSF1

Hintergrund:

Synonyms: HSTF1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1

Background: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11). Anti-HSF1 Antibody is ideal for research in Genetics, Transcription, Cell Signaling and pathways including ERK and MAPK.

Gene Name: Hsf1

Gen-ID: 15499

NCBI Accession: NP_058761

UniProt: P38532