Hemagglutinin Antikörper

Produktnummer ABIN6657575

Hersteller Produkt- Nr.: 200-301-976

Dieses Anti-Hemagglutinin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Hemagglutinin in ELISA, IP, WB, Neut und IHC. Geeignet für Influenza A Virus H5N1. Dieses Primary Antibody wurde in 6+ Publikationen zitiert.

Kurzübersicht für Hemagglutinin Antikörper (ABIN6657575)

Target: Hemagglutinin (HA)

Reaktivität: Influenza A Virus H5N1

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Hemagglutinin Antikörper ist unkonjugiert

Applikation: ELISA, Immunoprecipitation (IP), Western Blotting (WB), Neutralization (Neut), Immunohistochemistry (IHC)

Klon: 3G2

Produktdetails anti-Hemagglutinin Antikörper

Virus Strain: A/Vietnam/1203/04

Hersteller Produkt- Nr.: 200-301-976

Hersteller: Rockland

Verwendungszweck: H5N1 Antibody VN04-8

Kreuzreaktivität (Details): VN04-8 monoclonal antibody did not cross-react with influenza viruses of other HA subtypes.

Aufreinigung: This product was purified from tissue culture supernatant fluid by Protein A chromatography and is specific for H5 hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)].

Sterilität: Sterile filtered

Immunogen: Hemagglutinin of A/Vietnam/1203/04 Influenza Virus (VN04-8) monoclonal antibody was produced by intraperitoneal immunization of BALB/c mice with concentrated purified virus preparation containing hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)] using the modification of the method described by Kohler and Milstein. Each mouse received two immunizations of 15 mug HA with incomplete Freund's adjuvant, administered 3 week apart.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Neutralization_Dilution: User Optimized

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:5,000

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: User Optimized

Kommentare:

Suggested Applications: ELISA, IF, WB
Hemagglutinin of A/Vietnam/1203/04 Influenza Virus (VN04-8) monoclonal antibody can be used for hemagglutination inhibition (HI) assays to provide antigenic characterization of the influenza A viruses of the H5 HA subtype. This monoclonal antibody is suitable for virus neutralization assays (in cell culture and in embryonated chicken eggs), ELISA, immunoprecipitation, immunohistochemistry and western blotting.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-Hemagglutinin Antikörper (ABIN6657575)

Spieler, Moritz, Stertz, Hale: "Application of a Biologically Contained Reporter System To Study Gain-of-Function H5N1 Influenza A Viruses with Pandemic Potential." in: mSphere, Vol. 5, Issue 4, (2021) (PubMed).

Reed, Yen, DuBois, Bridges, Salomon, Webster, Russell: "Amino acid residues in the fusion peptide pocket regulate the pH of activation of the H5N1 influenza virus hemagglutinin protein." in: Journal of virology, Vol. 83, Issue 8, pp. 3568-80, (2009) (PubMed).

Kaverin, Rudneva, Govorkova, Timofeeva, Shilov, Kochergin-Nikitsky, Krylov, Webster: "Epitope mapping of the hemagglutinin molecule of a highly pathogenic H5N1 influenza virus by using monoclonal antibodies." in: Journal of virology, Vol. 81, Issue 23, pp. 12911-7, (2007) (PubMed).

Govorkova, Webby, Humberd, Seiler, Webster: "Immunization with reverse-genetics-produced H5N1 influenza vaccine protects ferrets against homologous and heterologous challenge." in: The Journal of infectious diseases, Vol. 194, Issue 2, pp. 159-67, (2006) (PubMed).

Hanson, Boon, Lim, Webb, Ooi, Webby: "Passive immunoprophylaxis and therapy with humanized monoclonal antibody specific for influenza A H5 hemagglutinin in mice." in: Respiratory research, Vol. 7, pp. 126, (2006) (PubMed).

Hoffmann, Lipatov, Webby, Govorkova, Webster: "Role of specific hemagglutinin amino acids in the immunogenicity and protection of H5N1 influenza virus vaccines." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 36, pp. 12915-20, (2005) (PubMed).

Details zu Hemagglutinin

Target: Hemagglutinin (HA)

Andere Bezeichnung: Hemagglutinin

Substanzklasse: Influenza Protein

Hintergrund:

Synonyms: mouse anti-H5N1 Antibody, mouse anti-VN04-8 Antibody, H5HA antibody, Hemagglutinin 5 antibody, H5N1 antibody

Background: Hemagglutinin of A/Vietnam/1203/04 Influenza Virus (VN04-8) Antibody raised against the hemagglutinin (HA) surface glycoprotein of the A/Vietnam/1203/04 (H5N1) influenza virus. Generally referred to as ''bird flu'', the H5N1 influenza A virus has been documented in poultry and humans across ten Eurasian countries, from Japan in the north to Indonesia in the south. Without immunity, humans would have no protection against H5N1 influenza viruses, which could potentially cause a catastrophic pandemic influenza.  This antibody, directed against the HA surface glycoprotein of the A/Vietnam/1203/04 (H5N1) influenza virus, is intended to further our understanding of the mechanisms underlying antigenic variation and evolution of novel variants. The major functions of HA include receptor-binding and fusion activities, but there may also be a structural role for HA in viral particle formation. Following attachment of HA to surface receptors on susceptible cells, the influenza virus enters the cell via endocytosis and membrane fusion.

Gene Name: HA

Gen-ID: 159144921

UniProt: A8UDQ2