CD34 Antikörper (C-Term)

Produktnummer ABIN6657527

Hersteller Produkt- Nr.: 600-401-p90

Der Kaninchen Polyklonal anti-CD34 Antikörper (ABIN6657527) detektiert spezifisch CD34 in FACS, WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CD34 Antikörper (C-Term) (ABIN6657527)

Target: CD34

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD34 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-CD34 Antikörper

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 600-401-p90

Hersteller: Rockland

Verwendungszweck: CD34 Antibody

Kreuzreaktivität (Details): Anti-CD34 antibody is directed against human CD34 protein.

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: CD34 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human CD34.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 0.5-1 μg/mL

Flow_Cytometry_Dilution: 1-3 μg/1x106 cells

Western_Blot_Dilution: 0.1-0.5 μg/mL

Other: Immunocytochemistry: 0.5-1 μg/mL

Kommentare:

Anti-CD34 is tested for Flow Cytometry, Immunohistochemistry-P, -F, Immunocytochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~40.7 kDa corresponding to the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu CD34

Target: CD34

Andere Bezeichnung: CD34

Hintergrund:

Synonyms: Hematopoietic progenitor cell antigen CD34, CD34 antibody

Background: CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30 % of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis. This antibody is suitable for researchers interested in stem cell, cancer and neuroscience research.

Gene Name: CD34

Gen-ID: 947

NCBI Accession: NP_001020280

UniProt: P28906