DDT Antikörper (C-Term)

Produktnummer ABIN6657521

Hersteller Produkt- Nr.: 600-401-r05

Dieses Anti-DDT-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von DDT in WB und IHC. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für DDT Antikörper (C-Term) (ABIN6657521)

Target: DDT (D-Dopachrome Tautomerase (DDT))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DDT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-DDT Antikörper

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 600-401-r05

Hersteller: Rockland

Verwendungszweck: DDT Antibody

Kreuzreaktivität (Details): Anti-DDT antibody is directed against human DDT protein.

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: DDT affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human DDT.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 0.5 μg/mL

Kommentare:

Suggested Applications: IP
Anti-DDT is tested for Immunohistochemistry-P and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~12.7 kDa corresponding to the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 200μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-DDT Antikörper (ABIN6657521)

Song, Liu, Habibie, van den Bor, Smit, Gosens, Wu, Brandsma, Cool, Haisma, Poelarends, Melgert: "D-dopachrome tautomerase contributes to lung epithelial repair via atypical chemokine receptor 3-dependent Akt signaling." in: EBioMedicine, Vol. 68, pp. 103412, (2021) (PubMed).

Details zu DDT

Target: DDT (D-Dopachrome Tautomerase (DDT))

Andere Bezeichnung: DDT

Hintergrund:

Synonyms: D-dopachrome tautomerase,Phenylpyruvate tautomerase II

Background: DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure. The genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10. This antibody is suitable for researchers interested in stem cell research.

Gene Name: DDT

Gen-ID: 1652

NCBI Accession: NP_001077861

UniProt: P30046