NOS1 Antikörper (AA 1-181)

Produktnummer ABIN6657480

Hersteller Produkt- Nr.: 200-301-p43

Dieses Maus Monoklonal-Antikörper erkennt spezifisch NOS1 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für NOS1 Antikörper (AA 1-181) (ABIN6657480)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NOS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: NOS-B1

Produktdetails anti-NOS1 Antikörper

Bindungsspezifität: AA 1-181

Hersteller Produkt- Nr.: 200-301-p43

Hersteller: Rockland

Verwendungszweck: Nitric Oxide Synthase (NOS1) Antibody

Kreuzreaktivität (Details): This product reacts with human, rat, goat, and pig.

Aufreinigung: Anti-NOS1 antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Nitric Oxide Synthase (NOS1) antibody was produced in mice by repeated immunizations with recombinant protein corresponding to neuronal NOS fragment (amino acids 1-181) from rat brain.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:5000

Western_Blot_Dilution: 0.5 μg/mL

Kommentare:

Anti-NOS1 is tested for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~161 kDa corresponding to the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Andere Bezeichnung: Nitric Oxide Synthase (NOS1)

Hintergrund:

Synonyms: NOS, bNOS, nNOS, IHPS1, N-NOS, NC-NOS, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1

Background: Nitric Oxide Synthase 1(NOS1,neuronal NOS,nNOS1) is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. It may be involved in neuronal cell death and damage in neurological illness. nNOS1 localized to the 12q24.2 region of human chromosome 12. It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098 amino acids. nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2. Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length. The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle. The novel gaseous neuromediator nitric oxide is thought to play an important role in development and plasticity. Despite this, gene-knockout mice lacking neuronal (Type I) nitric oxide synthase exhibit relatively normal brain development and behavior. This antibody is suitable for researchers interested in apoptosis research, cell death, and TNF pathways.

Gene Name: NOS1

Gen-ID: 4842

NCBI Accession: NP_000611

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction