EEF1A2 Antikörper (pSer358)

Produktnummer ABIN6657445

Produktdetails anti-EEF1A2 Antikörper

Target: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Bindungsspezifität: pSer358

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EEF1A2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität: Maus

Aufreinigung: Anti-Phospho-Ser358 eEF1A2 Antibody is directed against human eEF1A2 protein phosphorylated at S358. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated eEF1A2. Minimal reactivity occurs against non-phosphorylated eEF1A2. Reactivity against eEF1A2 occurs from mouse sources. However, reactivity is also expected against chicken, human, non-human primate, rat, sheep, and Xenopus based on 100% sequence homology.

Immunogen:

Immunogen: Anti-Phospho-Ser358 eEF1A2 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 of human eEF1A2 conjugated to KLH.

Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Phospho-Ser358 eEF1A2 Antibody is suitable for Western Blots and is specific for the ~50 kDa eEF1A2 protein phosphorylated at Ser358. Immunolabeling of the eEF1A2 band is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein). Specific conditions for reactivity should be optimized by the end user.

ELISA Dilution: 1:10,000

Western Blot Dilution: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Konservierungsmittel: Without preservative

Lagerung: RT,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilut

Details zu EEF1A2

Target: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Andere Bezeichnung: eEF1A2 (EEF1A2 Produkte)

Synonyme: EEF1AL antikoerper, EF-1-alpha-2 antikoerper, EF1A antikoerper, HS1 antikoerper, STN antikoerper, STNL antikoerper, EEF1A2 antikoerper, Eef1a antikoerper, S1 antikoerper, wasted antikoerper, wst antikoerper, Ps10 antikoerper, RATPS10 antikoerper, Stnl antikoerper, eef1al antikoerper, ef1a antikoerper, stnl antikoerper, EEF1A-2 antikoerper, zgc:92085 antikoerper, eukaryotic translation elongation factor 1 alpha 2 antikoerper, eukaryotic translation elongation factor 1 alpha 1 antikoerper, eukaryotic translation elongation factor 1 alpha 2 S homeolog antikoerper, putative elongation factor 1-alpha-like 3 antikoerper, EEF1A2 antikoerper, EEF1A1 antikoerper, Eef1a2 antikoerper, eef1a2.S antikoerper, eef1a2 antikoerper, LOC739210 antikoerper, EEF1 antikoerper, eef1 antikoerper

Hintergrund:

Synonyms: elongation factor 1-alpha 2, EF-1-alpha-2, Eukaryotic elongation factor 1 A-2, eEF1A-2, Statin-S1

Background: Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle. Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors. Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites. Ribosome-associated JNK phosphorylates Ser358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome. eEF1A2 pS358 Antibody is ideal for researchers interested in cancer research.

Gene Name: EEF1A2

Gen-ID: 1917

NCBI Accession: NP_001949

UniProt: Q05639