EEF1A2 Antikörper (pSer358)

Produktnummer ABIN6657445

Hersteller Produkt- Nr.: 600-401-x05

Der Kaninchen Polyklonal Anti-EEF1A2-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, EEF1A2 in Proben von Human zu detektieren.

Kurzübersicht für EEF1A2 Antikörper (pSer358) (ABIN6657445)

Target: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EEF1A2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-EEF1A2 Antikörper

Bindungsspezifität: pSer358

Hersteller Produkt- Nr.: 600-401-x05

Hersteller: Rockland

Verwendungszweck: eEF1A2 Phospho S358 Antibody

Kreuzreaktivität (Details): Anti-Phospho-Ser358 eEF1A2 Antibody is directed against human eEF1A2 protein phosphorylated at S358.

Aufreinigung: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen: Anti-Phospho-Ser358 eEF1A2 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 of human eEF1A2 conjugated to KLH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Phospho-Ser358 eEF1A2 Antibody has been tested by Western Blots and is specific for the ~50 kDa eEF1A2 protein phosphorylated at Ser358. Immunolabeling of the eEF1A2 band is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein). Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Haltbarkeit: 12 months

Details zu EEF1A2

Target: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Andere Bezeichnung: eEF1A2

Hintergrund:

Synonyms: elongation factor 1-alpha 2, EF-1-alpha-2, Eukaryotic elongation factor 1 A-2, eEF1A-2, Statin-S1

Background: Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle. Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors. Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites. Ribosome-associated JNK phosphorylates Ser358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome. eEF1A2 pS358 Antibody is ideal for researchers interested in cancer research.

Gene Name: EEF1A2

Gen-ID: 1917

NCBI Accession: NP_001949

UniProt: Q05639