ATRIP Antikörper (pSer239)

Produktnummer ABIN6657444

Hersteller Produkt- Nr.: 600-401-w95

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ATRIP in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ATRIP Antikörper (pSer239) (ABIN6657444)

Target: ATRIP (ATR Interacting Protein (ATRIP))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATRIP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-ATRIP Antikörper

Bindungsspezifität: pSer239

Hersteller Produkt- Nr.: 600-401-w95

Hersteller: Rockland

Verwendungszweck: ATRIP Phospho S239 Antibody

Kreuzreaktivität (Details): Anti-ATRIP pS239 Antibody is directed against human ATRIP protein phosphorylated at S239.

Aufreinigung: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen: Anti-Phospho-Ser239 ATRIP Antibody was produced from rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser239 of human ATRIP conjugated to KLH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:5,000

Western_Blot_Dilution: 1:500

Kommentare:

Anti-ATRIP Phospho-Ser239 Antibody is tested for WB. Expect a band at approximately ~86 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user. The antibody also recognizes a band at ~32k.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu ATRIP

Target: ATRIP (ATR Interacting Protein (ATRIP))

Andere Bezeichnung: ATRIP

Hintergrund:

Synonyms: ATM, Rad3-related-interacting protein, ATR-interacting protein

Background: ATRIP, ATR interacting protein, binds to ATR to regulate ATR expression, and is an essential component of the DNA damage checkpoint pathway (Cortez et al, 2001). ATR is recruited to DNA lesions in part through its association with ATRIP, which in turn interacts with the single-stranded DNA binding protein RPA (Ball et al, 2007). DNA replication forks may stall as a result of DNA damage causing phosphorylation of several proteins, including BRCA1 when colocalizing with ATR/ATRIP complex and RPA (Venere et al, 2007). The DNA replication fork stall coincides with BRCA1 directly phosphorylating ATRIP at ser239. ATRIP pS239 Antibody is ideal for researchers interested in cancer research.

Gene Name: ATRIP

Gen-ID: 84126

NCBI Accession: NP_001257951

UniProt: Q8WXE1