CYP1A1 Antikörper

Produktnummer ABIN6657370

Hersteller Produkt- Nr.: 600-401-hb7s

Der Kaninchen Polyklonal Anti-CYP1A1-Antikörper wurde für WB, ELISA, IHC, IF, FM und MA validiert. Er ist geeignet, CYP1A1 in Proben von Human zu detektieren.

Kurzübersicht für CYP1A1 Antikörper (ABIN6657370)

Target: CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CYP1A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Produktdetails anti-CYP1A1 Antikörper

Hersteller Produkt- Nr.: 600-401-hb7s

Hersteller: Rockland

Verwendungszweck: Cytochrome P450 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human cytochrome p450.

Aufreinigung: This product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-Cytochrome p450 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human cytochrome p450 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 5 μg/mL

Immunohistochemistry_Dilution: 1:100 - 1:200

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Cytochrome p450 has been tested in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Expect a band at ~58kda in western blot using appropriate lysates. Positive controls used include an overexpressing lysate and PC-12 at 1μg/mL for WB, human kidney and/or lung for IHC, HepG2 for IF at 15 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CYP1A1

Target: CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))

Andere Bezeichnung: CYP1A1

Hintergrund:

Synonyms: Rabbit Anti-Cytochrome p450 Antibody, Rabbit Anti-CYP1A1 Antibody, CYPIA1, Cytochrome P450 form 6, Cytochrome P450-C, Cytochrome P450-P1

Background: Cytochrome p450 belongs to an enzyme superfamily that catalyzes many reactions such as the synthesis of cholesterol, lipids, steroids by oxidizing the compounds and plays a role in drug metabolism. The protein has been known to be stimulated by cigarette smoke. On-going studies link the cytochrome p450 protein with lung cancer. In the liver microsome, it is part of the NADPH-dependent electron transport pathway. The protein has been found expressed in the lung, lymphocytes and placenta. Anti-Cytochrome p450 antibody is useful for researchers interested in cancer research and enzyme research.

Gene Name: CYP1A1

Gen-ID: 1543

NCBI Accession: NP_000490

UniProt: P04798

Pathways: Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha