PCLO Antikörper

Produktnummer ABIN6657340

Hersteller Produkt- Nr.: 600-401-g30

Der Kaninchen Polyklonal Anti-PCLO-Antikörper wurde für WB, IHC und FM validiert. Er ist geeignet, PCLO in Proben von Human zu detektieren.

Kurzübersicht für PCLO Antikörper (ABIN6657340)

Target: PCLO (Piccolo (Presynaptic Cytomatrix Protein) (PCLO))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PCLO Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Produktdetails anti-PCLO Antikörper

Hersteller Produkt- Nr.: 600-401-g30

Hersteller: Rockland

Verwendungszweck: Piccolo Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Piccolo from Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Piccolo Antibody was purified by affinity chromatography.

Sterilität: Sterile filtered

Immunogen: Piccolo Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against full length protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IF_Microscopy_Dilution: 1 μg/mL

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Piccolo Antibody is tested for use in WB and IHC. Expect a band approximately ~550kDa corresponding to the molecular weight of Piccolo. Multiple isoforms can be detected. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu PCLO

Target: PCLO (Piccolo (Presynaptic Cytomatrix Protein) (PCLO))

Andere Bezeichnung: PCLO

Hintergrund:

Synonyms: Protein piccolo, Aczonin, PCLO, ACZ, KIAA0559

Background: Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains. In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1.

Gene Name: PCLO

Gen-ID: 27445

NCBI Accession: NP_055325

UniProt: Q9Y6V0

Pathways: Hormone Transport, Synaptic Vesicle Exocytosis