AQP2 Antikörper (C-Term)

Produktnummer ABIN6656925

Hersteller Produkt- Nr.: 600-401-e90

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch AQP2 in WB, IHC, IF und FM. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für AQP2 Antikörper (C-Term) (ABIN6656925)

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AQP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Produktdetails anti-AQP2 Antikörper

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 600-401-e90

Hersteller: Rockland

Verwendungszweck: Aquaporin 2 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Aquaporin 2 from Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Aquaporin 2 Antibody was purified by affinity chromatography.

Sterilität: Sterile filtered

Immunogen: Aquaporin 2 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of rat aquaporin 2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 1:200

IF_Microscopy_Dilution: 1:400

Western_Blot_Dilution: 1:1000 - 1:4000

Kommentare:

Anti-Aquaporin 2 Antibody is tested for use in WB, IHC, and IF microscopy. Expect a band approximately ~28.5kDa on specific lysates. May detect larger glycosylated bands ~35-50kDa. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu AQP2

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Andere Bezeichnung: Aquaporin 2

Hintergrund:

Synonyms: AQP2, AQP-CD, AQPCD, WCH CD, Aquaporin-2, AQP-2, ADH water channel, Aquaporin-CD, QP-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct

Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins. Aquaporin 2 is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. It is located in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed vasopressin binds to the cell surface vasopressin receptor, activating a signaling pathway that cause AQP2 containing vesicles to fuse with the plasma membrane so the AQP2 can be used by the cell. Defects in AQP2 area cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI).

Gene Name: Aqp2

Gen-ID: 25386

NCBI Accession: NP_037041

UniProt: P34080

Pathways: Response to Water Deprivation