Rab4 Antikörper (C-Term)

Produktnummer ABIN6656358

Hersteller Produkt- Nr.: 100-401-g35

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Rab4 in WB, IHC und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rab4 Antikörper (C-Term) (ABIN6656358)

Target: Rab4 (RAB4A) (RAB4A, Member RAS Oncogene Family (RAB4A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Rab4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-Rab4 Antikörper

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 100-401-g35

Hersteller: Rockland

Verwendungszweck: RAB4 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Rab4 from Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Rab4 Antibody was prepared from monospecific antiserum by delipidation and defibrination.

Sterilität: Sterile filtered

Immunogen: Rab4 Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against a C-terminal region synthetic peptide from human Rab4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 1:1000-2000

Kommentare:

Anti-Rab4 Antibody is tested by WB, IHC, and IF microscopy. Expect a band approximately ~26kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Rab4

Target: Rab4 (RAB4A) (RAB4A, Member RAS Oncogene Family (RAB4A))

Andere Bezeichnung: Rab4

Hintergrund:

Synonyms: Oncogene Rab4, Rab4A, Ras related protein Rab-4A

Background: Rab4 is a 25 kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a sub-cellular compartment. Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion. Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper-variable COOH-terminal domains with a cysteine motif implicated in sub-cellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins. Each Rab shows a characteristic sub-cellular distribution. In particular, over-expression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. The presence of excessive Rab4 leads to the accumulation of tranferrin receptors in non-acidic, post-endosomal recycling vesicles considered an intermediate compartment between endosomes and plasma membranes. Rab4 also plays a role in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin. Mediating the association of Rab4 with transferring receptor-containing early endosomes takes place through the geranylgeranyl groups at its carboxyl-terminus. Membrane association is also cell cycle dependent, as phosphorylation at its c-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol.

Gene Name: RAB4A

Gen-ID: 5867

NCBI Accession: NP_004569

UniProt: P20338