EZH2 Antikörper (N-Term)

Produktnummer ABIN6656040

Hersteller Produkt- Nr.: 200-401-w53

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch EZH2 in WB, IHC, IF und ChIP. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für EZH2 Antikörper (N-Term) (ABIN6656040)

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EZH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)

Produktdetails anti-EZH2 Antikörper

Bindungsspezifität: N-Term

Hersteller Produkt- Nr.: 200-401-w53

Hersteller: Rockland

Verwendungszweck: EzH2 Antibody

Kreuzreaktivität (Details): Cross reactivity with other species was not tested.

Aufreinigung: Anti-EZH2 Antibody was purified by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: Anti-EZH2 Antibody was produced in rabbits by repeated immunizations containing an amino acid sequence from the N-terminus of mouse EZH2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ChIP_Dilution: 2.5 μg per IP

Immunohistochemistry_Dilution: 1:100 - 1:500

Western_Blot_Dilution: 1:1,000

Kommentare:

Anti-EZH2 Antibody is tested for Chromatin Immunoprecipitation, Immunofluorescence, and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 85 kDa in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

Konservierungsmittel: ProClin, Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu EZH2

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Andere Bezeichnung: EzH2

Hintergrund:

Synonyms: Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2

Background: EZH2 is a histone-lysine methyltransferase which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression. It is a member of the polycomb group (PcG) family which form multimeric protein complexes and are involved in maintaining the transcriptional repressive state of genes over successive cell generations. The EZH2 activity is dependent on the association with other components of the PRC2 complex (EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7). EZH2 may play a role in the hematopoietic and internal nervous systems. Over-expression of EZH2 is observed during advanced stages of prostate cancer and breast cancer. Anti-EZH2 Antibody is ideal for research in Epigenetics, Gene Expression, Cell Biology and Cancer.

Gene Name: EZH2

Gen-ID: 2146

NCBI Accession: NP_001190176

UniProt: Q15910

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation