NOXA Antikörper

Produktnummer ABIN6655871

Hersteller Produkt- Nr.: 200-301-h98

Dieses Anti-NOXA-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von NOXA in WB, IF, IHC und FACS. Geeignet für Human. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für NOXA Antikörper (ABIN6655871)

Target: NOXA (PMAIP1) (Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NOXA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Klon: 114C307-1

Produktdetails anti-NOXA Antikörper

Hersteller Produkt- Nr.: 200-301-h98

Hersteller: Rockland

Verwendungszweck: NOXA Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Noxa from human and mouse based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Noxa Antibody was purified by Protein G chromatography.

Immunogen: Noxa Antibody was produced in mice prepared by repeated immunizations with the human protein Noxa.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 5 μg/mL

Western_Blot_Dilution: 1-2 μg/mL

Kommentare:

Anti-Noxa antibody is tested for use by WB, Flow, ICC/IF, IHC, and IHC-P. Expect a band approximately 6 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-NOXA Antikörper (ABIN6655871)

Haschka, Karbon, Soratroi, O'Neill, Luo, Villunger: "MARCH5-dependent degradation of MCL1/NOXA complexes defines susceptibility to antimitotic drug treatment." in: Cell death and differentiation, Vol. 27, Issue 8, pp. 2297-2312, (2021) (PubMed).

Ichikawa, Alves, Pfeiffer, Langa, Hernández-Santana, Suzuki, Prehn, Engel, Henshall: "Deletion of the BH3-only protein Noxa alters electrographic seizures but does not protect against hippocampal damage after status epilepticus in mice." in: Cell death & disease, Vol. 8, Issue 1, pp. e2556, (2017) (PubMed).

Details zu NOXA

Target: NOXA (PMAIP1) (Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1))

Andere Bezeichnung: Noxa

Hintergrund:

Synonyms: NOXA, Phorbol-12-myristate-13-acetate-induced protein 1, PMA-induced protein 1, Immediate-early-response protein APR, Protein Noxa

Background: Anti-Noxa antibody detects human Noxa. Recently, a BH-3 only member of the Bcl-2 family have been identified in human and mouse and designated as Noxa (for damage). The expression of the Noxa gene involves direct activation of its promoter by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Noxa cDNA codes for a 103-amino acid protein. The coimmunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl-2 family, such as, Bcl-XL and Mcl-1. Oda et al. have also shown that blocking the endogenous Noxa induction results in the suppression of apoptosis. Treatment of cells with Noxa antisense oligonucleotides blocks radiation-induced apoptosis suggesting that Noxa may represent a mediator of p53-dependent apoptosis. Anti-Noxa antibody is ideal for investigators involved in apaoptosis research.

Gene Name: PMAIP1

Gen-ID: 5366

NCBI Accession: NP_066950

UniProt: Q13794

Pathways: p53 Signalweg, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus