DNMT1 Antikörper

Produktnummer ABIN6655855

Hersteller Produkt- Nr.: 200-301-h69

Der Maus Monoklonal Anti-DNMT1-Antikörper wurde für WB, IHC, ELISA, IP, IF, ChIP und FACS validiert. Er ist geeignet, DNMT1 in Proben von Human zu detektieren.

Kurzübersicht für DNMT1 Antikörper (ABIN6655855)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DNMT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS)

Klon: 60B1220-1

Produktdetails anti-DNMT1 Antikörper

Hersteller Produkt- Nr.: 200-301-h69

Hersteller: Rockland

Verwendungszweck: DNMT1 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Dnmt1 from human, mouse and Zebrafish based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Dnmt1 Antibody was purified by Protein G chromatography.

Immunogen: Dnmt1 Antibody was produced in mice prepared by repeated immunizations with a synthetic peptide corresponding to internal amino acids with Dnmt1 human protein.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 0.1-0.5 μg/mL

Immunohistochemistry_Dilution: 1-2 μg/mL

Flow_Cytometry_Dilution: 1-5 μg/mL

Western_Blot_Dilution: 0.1-0.5 μg/mL

Kommentare:

Anti-DNMT1 antibody is tested for use in WB, ChIP, Flow, Flow-IC, ICC/IF, IHC-Fr, IHC-P, and IP. Expect a band approximately 183 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Andere Bezeichnung: Dnmt1

Hintergrund:

Synonyms: AIM, CXXC9, DNMT, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI

Background: Anti-Dnmt1 antibody detects human Dnmt1. Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. Anti-Dnmt1 antibody is ideal for investigators involved in cancer, cell cycle protein research and epigenetics.

Gene Name: DNMT1

Gen-ID: 1786

NCBI Accession: NP_001124295

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion