DGCR8 Antikörper (pSer377)

Produktnummer ABIN6655713

Hersteller Produkt- Nr.: 600-401-x01

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch DGCR8 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für DGCR8 Antikörper (pSer377) (ABIN6655713)

Target: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DGCR8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-DGCR8 Antikörper

Bindungsspezifität: pSer377

Hersteller Produkt- Nr.: 600-401-x01

Hersteller: Rockland

Verwendungszweck: DGCR8 Phospho S377 Antibody

Aufreinigung: Anti-Phospho-Ser377 DGCR8 Antibody was prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Sterilität: Sterile filtered

Immunogen: Anti-Phospho-Ser377 DGCR8 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser377 of human DGCR8 conjugated to KLH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Phospho-Ser377 DGCR8 Antibody is tested for Western Blots and is specific for the ~120 kDa DGCR8 protein phosphorylated at Ser377. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu DGCR8

Target: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Andere Bezeichnung: DGCR8

Hintergrund:

Synonyms: Microprocessor complex subunit DGCR8, DiGeorge syndrome critical region 8, C22orf12, DGCRK9

Background: The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away. The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA. DGCR8 pS377 Antibody is ideal for researchers interested in neuroscience research.

Gene Name: DGCR8

Gen-ID: 1644

NCBI Accession: NP_001177255

UniProt: Q8WYQ5

Pathways: Regulatorische RNA Pathways