Tumor Protein p73 Antikörper

Produktnummer ABIN6655655

Hersteller Produkt- Nr.: 200-301-i00

Dieses Anti-Tumor Protein p73-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Tumor Protein p73 in WB, IHC, IF, IP, FACS und ChIP. Geeignet für Human.

Kurzübersicht für Tumor Protein p73 Antikörper (ABIN6655655)

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Tumor Protein p73 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Chromatin Immunoprecipitation (ChIP)

Klon: 5B429

Produktdetails anti-Tumor Protein p73 Antikörper

Hersteller Produkt- Nr.: 200-301-i00

Hersteller: Rockland

Verwendungszweck: p73 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-p73 from Human and Mouse based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-p73 Antibody was purified by Protein G chromatography.

Immunogen: p73 Antibody was produced in mice prepared by repeated immunizations with full-length human p73 protein.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 5-15 μg/mL

Western_Blot_Dilution: 2 μg/mL

Kommentare:

Anti-p73 Antibody is tested for use in WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, and IP. Expect a band approximately 70kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Tumor Protein p73

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Andere Bezeichnung: p73

Hintergrund:

Synonyms: P73, Tumor protein p73, p53-like transcription factor, p53-related protein

Background: Anti-p73 detects human p73. NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. External stimuli such as tumor necrosis factor or other cytokines initiates a signal transduction cascade that leads to the activation of IkB-kinase complex that specifically phosphorylates IkBa on Serine-32 and Serine-36. Phosphorylation of these sites leads to ubiquitination of IkBa and subsequent degradation by the 26 S proteasome. Degradation of IkBa results in unmasking of the nuclear localization signal of NF-kB dimers, which subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified. One of the first gene induced following NF-kB activation is IkBa. Anti-p73 antibody is ideal for investigators involved in NFkappaB, kinase and phosphatse research.

Gene Name: TP73

Gen-ID: 7161

NCBI Accession: NP_001119712

UniProt: O15350

Pathways: Regulation of Cell Size, Positive Regulation of Response to DNA Damage Stimulus